JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB265587

Anti-SOX10 antibody [BLR080G]

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Recombinant Monoclonal SOX10 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SOX10 aa 400-500.

대체 명칭 보기

Transcription factor SOX-10, SOX10

6 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [BLR080G] (AB265587)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [BLR080G] (AB265587)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human melanoma tissue labeling SOX10 with ab265587 at 1/100 dilution. Detection : DAB.

Immunocytochemistry - Anti-SOX10 antibody [BLR080G] (AB265587)
  • ICC

Supplier Data

Immunocytochemistry - Anti-SOX10 antibody [BLR080G] (AB265587)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded SK-MEL-28 (Human melanoma cell line) cells labeling SOX10 with ab265587 at 1/100 dilution. Detection : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [BLR080G] (AB265587)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [BLR080G] (AB265587)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling SOX10 with ab265587 at 1/100 dilution. Detection : DAB.

Immunoprecipitation - Anti-SOX10 antibody [BLR080G] (AB265587)
  • IP

Supplier Data

Immunoprecipitation - Anti-SOX10 antibody [BLR080G] (AB265587)

SOX10 was immunoprecipitated from 1mg of MALME-3M whole cell lysate with ab265587 at 20 μl/mg lysate. Western blot was performed from the immunoprecipitate using ab265587 at a 1/1000 dilution.
Lane 1 : ab265587 IP in MALME-3M whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 3 secs.

Cells prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-SOX10 antibody [BLR080G] (ab265587)

Predicted band size: 49 kDa

false

Western blot - Anti-SOX10 antibody [BLR080G] (AB265587)
  • WB

Supplier Data

Western blot - Anti-SOX10 antibody [BLR080G] (AB265587)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-SOX10 antibody [BLR080G] (ab265587) at 1/1000 dilution

Lane 1:

HEK-293T whole cell lysate at 15 µg

Lane 2:

MALME-3M whole cell lysate at 15 µg

Lane 3:

MCF7 whole cell lysate at 15 µg

Lane 4:

SK-MEL-28 whole cell lysate at 15 µg

Lane 5:

Jurkat whole cell lysate at 15 µg

Lane 6:

MDA-MB-435 whole cell lysate at 15 µg

Lane 7:

HeLa whole cell lysate at 15 µg

Lane 8:

UACC-62 whole cell lysate at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 49 kDa

false

Exposure time: 10s

Western blot - Anti-SOX10 antibody [BLR080G] (AB265587)
  • WB

Supplier Data

Western blot - Anti-SOX10 antibody [BLR080G] (AB265587)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-SOX10 antibody [BLR080G] (ab265587) at 1/1000 dilution

Lane 1:

TCMK whole cell lysate at 50 µg

Lane 2:

B16-F10 whole cell lysate at 15 µg

Lane 3:

NIH/3T3 whole cell lysate at 50 µg

Lane 4:

MALME-3M whole cell lysate at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 49 kDa

false

Exposure time: 3s

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR080G

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, IHC-P, ICC, WB

applications

Immunogen

Synthetic Peptide within Human SOX10 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

P56693

style
left-column

대체품

Primary Antibodies

AB155279

Anti-SOX10 antibody [EPR4007]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007] (AB155279)

  • 4
  • 7
Primary Antibodies

AB220078

Anti-SOX10 antibody [EPR4007-104] - BSA and Azide free

BOND RX™ Validated|Recombinant|Advanced Validation|RabMAb

Multiplex immunohistochemistry - Anti-SOX10 antibody [EPR4007-104] - BSA and Azide free (AB220078)

  • 4
  • 3
Primary Antibodies

AB227680

Anti-SOX10 antibody [SP267]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (AB227680)

  • 5
  • 9
Primary Antibodies

AB239751

Anti-SOX10 antibody [SP275] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP275] - BSA and Azide free (AB239751)

  • 0
  • 0
Primary Antibodies

AB245760

Anti-SOX10 antibody [SP267] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] - BSA and Azide free (AB245760)

  • 0
  • 0
Primary Antibodies

AB180862

Anti-SOX10 antibody [EPR4007-104]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Multiplex immunohistochemistry - Anti-SOX10 antibody [EPR4007-104] (AB180862)

  • 5
  • 10

보완 제품

Primary Antibodies

AB181560

Anti-Pan Trk antibody [EPR17341]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (AB181560)

  • 5
  • 1
Primary Antibodies

AB133273

Anti-Androgen Receptor antibody [EPR1535(2)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

  • 5
  • 12
Primary Antibodies

AB170933

Anti-FOXA1 + FOXA2 + FOXA3 antibody [EPR10881]

RabMAb|Recombinant|Advanced Validation|20ul selling size

Flow Cytometry (Intracellular) - Anti-FOXA1 + FOXA2 + FOXA3 antibody [EPR10881] (AB170933)

  • 5
  • 1
Assay Kits

AB65330

L-Lactate Assay Kit (Colorimetric/Fluorometric)

Biochemical assay - L-Lactate Assay Kit (Colorimetric/Fluorometric) (AB65330)

  • 4
  • 3
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "<p>Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with ICC staining protocol.</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20 μl per mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Purified from TCS.
보관 버퍼
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed : 21965087). Transcriptional activator of MBP, via binding to the gene promoter (By similarity).
See full target information SOX10
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in molecular biosciences 8:729789 PubMed34621787

2021

Development of a Toll-Like Receptor-Based Gene Signature That Can Predict Prognosis, Tumor Microenvironment, and Chemotherapy Response for Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Liu,Bin Liu,Jie Yu,Dongyun Zhang,Jianhong Shi,Ping Liang
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com