Anti-SOX11 antibody [EPR8192]

• IP

Lab

Immunoprecipitation - Anti-SOX11 antibody [EPR8192] (AB134107)

SOX11 was immunoprecipitated from 0.35 mg Y79 (Human retinoblastoma retinoblastoma) whole cell lysate 10 μg with 134107 at 1/40 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Y79 (Human retinoblastoma retinoblastoma) whole cell lysate 10 μg

Lane 2 : ab134107 IP in Y79 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab134107 in Y79 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-SOX11 antibody [EPR8192] (ab134107)

Predicted band size: 47 kDa

Observed band size: 60 kDa

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• WB

Unknown

Western blot - Anti-SOX11 antibody [EPR8192] (AB134107)

All lanes:

Western blot - Anti-SOX11 antibody [EPR8192] (ab134107) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Y-79 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 47 kDa

Observed band size: 60 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SOX11 antibody [EPR8192] (AB134107)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.