AB239218

Anti-SOX2 antibody

Be the first to review this product! 리뷰 제출

(6 제품이 사용된 논문 )

Anti-SOX2 antibody (ab239218) is a goat polyclonal antibody detecting SOX2 in Western Blot, Flow Cytometry (Intra), ICC/IF. Suitable for Human.

대체 명칭 보기

Transcription factor SOX-2, SOX2

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (AB239218)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (AB239218)

Immunofluorescence analysis of paraformaldehyde fixed U-251 MG (Human brain glioma cell line) cells, permeabilized with 0.15% Triton, labeling SOX2 (green) using ab239218. Primary incubation time 1 hour (10 μg/ml) followed by an Alexa-Fluor^®488 secondary antibody (2 μg/ml). Actin filaments were stained with phalloidin (red).

Negative control : Non-immunized goat IgG (10 μg/ml) followed by an Alexa-Fluor^®488 secondary antibody (2 μg/ml).

Flow Cytometry (Intracellular) - Anti-SOX2 antibody (AB239218)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SOX2 antibody (AB239218)

Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr using ab239218 (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Supplier Data

Western blot - Anti-SOX2 antibody (AB239218)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-SOX2 antibody (ab239218) at 0.3 µg/mL

Lane 1:

MCF-7 cell lysates at 35 µg

Lane 2:

HEK293 cell lysates at 35 µg

Lane 3:

MCF7 nuclear cell lysates at 35 µg

Predicted band size: 34 kDa

false

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

Synthetic Peptide within Human SOX2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P48431

style

left-column

대체품

Primary Antibodies

AB92494

Anti-SOX2 antibody [EPR3131]

RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (AB92494)

Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

Primary Antibodies

AB196637

Alexa Fluor® 647 Anti-SOX2 antibody [EPR3131]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SOX2 antibody [EPR3131] (AB196637)

Primary Antibodies

AB215970

Anti-SOX2 antibody [EPR3131] - BSA and Azide free

RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (AB215970)

Primary Antibodies

AB243909

Anti-SOX2 antibody [SP76] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [SP76] - BSA and Azide free (AB243909)

보완 제품

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB19857

Anti-Oct4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB19857)

Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

제품 세부 정보

What is this antibody validated in?
Anti-SOX2 antibody (ab239218) is a goat polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed : 18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).

See full target information SOX2

style

left-column

제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in molecular neuroscience 18:1552819 PubMed40206188

2025

The restoration of REST inhibits reactivity of Down syndrome iPSC-derived astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tan Huang,Sharida Fakurazi,Pike-See Cheah,King-Hwa Ling

STAR protocols 6:103633 PubMed39932849

2025

Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Shuhui Sun,Juan Carlos Izpisua Belmonte,Guang-Hui Liu,Si Wang,Weiqi Zhang,Jing Qu

Scientific reports 15:2818 PubMed39843579

2025

Dysregulation of REST and its target genes impacts the fate of neural progenitor cells in down syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Tan Huang,Sharida Fakurazi,Pike-See Cheah,King-Hwa Ling

Frontiers in cell and developmental biology 11:1270892 PubMed37928906

2023

Transcriptome analysis reveals genes associated with stem cell activation by physical exercise in the dentate gyrus of aged p16Ink4a knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Micheli,Giorgio D'Andrea,Teresa Maria Creanza,Daniel Volpe,Nicola Ancona,Raffaella Scardigli,Felice Tirone

Neurotoxicity research 40:1057-1069 PubMed35699893

2022

Geniposide Alleviates Neuropathic Pain in CCI Rats by Inhibiting the EGFR/PI3K/AKT Pathway And Ca Channels.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Zhang,Qiao-Qiao Chen,Li Yao

Frontiers in cell and developmental biology 9:696684 PubMed34485283

2021

Transcriptome Analysis in a Mouse Model of Premature Aging of Dentate Gyrus: Rescue of Alpha-Synuclein Deficit by Virus-Driven Expression or by Running Restores the Defective Neurogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Micheli,Teresa Maria Creanza,Manuela Ceccarelli,Giorgio D'Andrea,Giacomo Giacovazzo,Nicola Ancona,Roberto Coccurello,Raffaella Scardigli,Felice Tirone

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com