AB13370

Anti-SP1 antibody

Name: Anti-SP1 antibody
Brand: Abcam Limited
SKU: AB13370
Rating: 4 (13 reviews)

(13 리뷰들)

(103 제품이 사용된 논문 )

Rabbit Polyclonal SP1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 103 publications. Immunogen corresponding to Synthetic Peptide within Human SP1.

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (AB13370)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (AB13370)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling SP1 with ab13370 at 1/5000 dilution. DAB detection.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (AB13370)

ab13370 (2µg/ml) staining SP1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Supplier Data

Immunoprecipitation - Anti-SP1 antibody (AB13370)

SP1 was immunoprecipitated from HeLa cells whole cell lysate with ab13370. Anti-SP1 antibody - ChIP Grade (ab13370) used at 6μg per reaction. SP1 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated SP1, ab13370 was used at 1μg/ml. Chemiluminescence with an exposure time of 10 seconds.

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Immunoprecipitation - Anti-SP1 antibody (ab13370)

Predicted band size: 81 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (AB13370)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue sections labeling SP1 with ab13370 at 1/5000 dilution. DAB detection.

Unknown

Western blot - Anti-SP1 antibody (AB13370)

Detection of Human SP1 by Western Blot and Immunoprecipitation. Samples : A. Whole cell lysate (50 mcg for E; 10 mcg for T) from HEK 293T cells that were mock transfected (E) or transfected with a Sp1 expression construct (T). B. Whole cell lysate (700 mcg) from HEK 293T cells. Antibody : A. Affinity purified rabbit anti-Sp1 antibody (ab13370) used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies (lane 2 : ab13405, lane 3 : ab13370; lane 1 : SP-1 antibody, details unknown) using each antibody at 3.75 mcg/mg lysate. Subsequently, ab13370 was used at 0.04 mcg/ml for WB. Detection : Chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).

All lanes:

Western blot - Anti-SP1 antibody (ab13370)

Predicted band size: 81 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SP1. The exact immunogen used to generate this antibody is proprietary information.

P08047

style

left-column