AB308364

Anti-SP1 antibody [HL1396]

Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal SP1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat, Transfected cell line samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SP1.

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Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of human breast carcinoma tissue labeling SP1 using ab308364 at 1/500 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of human breast carcinoma tissue labeling SP1 using ab308364 at 1/500 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (AB308364)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (AB308364)

For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SP1 protein using ab308364 at 1/500 dilution (green). Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of rat intestine, colon and lung tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of mouse intestine, colon and lung tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of mouse intestine tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1396

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SP1. The exact immunogen used to generate this antibody is proprietary information.

P08047

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Also binds the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component BMAL1 (PubMed : 10391891, PubMed : 11371615, PubMed : 11904305, PubMed : 14593115, PubMed : 16377629, PubMed : 16478997, PubMed : 16943418, PubMed : 17049555, PubMed : 18171990, PubMed : 18199680, PubMed : 18239466, PubMed : 18513490, PubMed : 18619531, PubMed : 19193796, PubMed : 20091743, PubMed : 21046154, PubMed : 21798247). Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (By similarity).

See full target information SP1

대체 명칭 보기

TSFP1, SP1, Transcription factor Sp1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

BMC cancer 25:737 PubMed40254586

2025

Targeting GSK3β and signaling pathways in breast cancer: role of individual members of miR- 23/24/27 cluster.

Applications

Unspecified application

Species

Unspecified reactive species

Harshi Gupta,Anushka Raghubansi, Bharat,Kritika Sharma,Krittika Zutshi,Partibha Panchal,Sushant Bhattacharya,Piyush Ranjan,Gopal Puri,Neeru Saini

Folia biologica 70:219-228 PubMed39692576

2024

Effects of Different Light Environments with Varying Spectral Composition on the Axial Lengths and Scleral Specificity Protein 1 and Collagen Type I Expression in Juvenile Guinea Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbao Yuan,Yuliang Wang,Xinyu Xu,Mei Yang,Yipeng Fan,Xiaopan Shi,Lulu Sun,Mingyu Shan,Lei Ma

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SP1 antibody [HL1396]

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise