Anti-SPACA1 antibody - C-terminal

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SPACA1 antibody - C-terminal (AB191843)

Immunofluorescent analysis of round spermatids labeling SPACA1 with ab191843 at 1/100 and as a secondary antibody, Alexa-Fluor 488 conjugated anti-rabbit IgG antibody. Image at the right stained with Hoechst 33342.

• WB

Supplier Data

Western blot - Anti-SPACA1 antibody - C-terminal (AB191843)

Basigin was used as a loading control.

All lanes:

Western blot - Anti-SPACA1 antibody - C-terminal (ab191843) at 1/1000 dilution

Lane 1:

extracts from testis of wild type (+/+) mice

Lane 2:

extracts from testis of SPACA1 single (+/-) knock-out mice

Lane 3:

extracts from testis of SPACA1 double (-/-) knock-out mice

Predicted band size: 32 kDa

false

• WB

Supplier Data

Western blot - Anti-SPACA1 antibody - C-terminal (AB191843)

Several proteins associated with sperm undergo modification during maturation. Multiple bands are reflection of post-translational modifications by glycosylation and phosphorylation.

All lanes:

Western blot - Anti-SPACA1 antibody - C-terminal (ab191843) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate

Lane 2:

Mouse sperm cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 32 kDa

false