AB121395

Anti-SPCS2/SPC25 antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal SPCS2/SPC25 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPCS2 aa 1-100.

대체 명칭 보기

KIAA0102, SPC25, SPCS2, Signal peptidase complex subunit 2, Microsomal signal peptidase 25 kDa subunit, SPase 25 kDa subunit

8 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB121395)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB121395)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling SPCS2/SPC25 with ab121395 at 1/50 dilution. Heat induced epitope retrieval pH 6 performed before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB121395)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling SPCS2/SPC25 with ab121395 at 1/50 dilution. Heat induced epitope retrieval pH 6 performed before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-SPCS2/SPC25 antibody (AB121395)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SPCS2/SPC25 antibody (AB121395)

Immunocytochemistry/immunofluorescence analysis of human cell line U-2 OS labelling SPCS2/SPC25 with ab121395 at 2 μg/mL

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB121395)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human epididymis tissue labelling SPCS2/SPC25 with ab121395 at 1/50 dilution. Heat induced epitope retrieval pH 6 performed before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB121395)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling SPCS2/SPC25 with ab121395 at 1/50 dilution. Heat induced epitope retrieval pH 6 performed before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB121395)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling SPCS2/SPC25 with ab121395 at 1/50 dilution. Heat induced epitope retrieval pH 6 performed before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SPCS2/SPC25 antibody (AB121395)

All lanes:

Western blot - Anti-SPCS2/SPC25 antibody (ab121395) at 1/250 dilution

Lane 1:

RT-4

Lane 2:

U-251 MG

Lane 3:

Human plasma

Lane 4:

Human liver

Lane 5:

Human tonsil

Predicted band size: 25 kDa

true

Supplier Data

Western blot - Anti-SPCS2/SPC25 antibody (AB121395)

All lanes:

Western blot - Anti-SPCS2/SPC25 antibody (ab121395) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryonic fibroblasts) cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumour) cell lysate

Predicted band size: 25 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SPCS2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q15005

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.2-0.25 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SPCS2 also called SPC25 is a component of the signal peptidase complex involved in cleaving signal peptides from nascent proteins during translocation into the endoplasmic reticulum. This protein has a molecular mass of approximately 27 kDa. SPCS2 is expressed in various tissues with notable levels in the liver pancreas and kidney. This broad expression suggests its importance across multiple physiological systems.

Biological function summary

The function of SPCS2/SPC25 relates to its role in the signal peptidase complex. It participates in the maturation of proteins by processing them for proper folding and export within the secretory pathway. As part of this multimeric complex in the endoplasmic reticulum membrane SPCS2 coordinates with other components like SEC11 to efficiently cleave signal peptides. Its function is critical for the regulation of protein synthesis and secretion processes.

Pathways

SPCS2 functions within the secretory pathways particularly in protein translocation into the endoplasmic reticulum. Its involvement in the ER-associated degradation pathway highlights its role in maintaining protein homeostasis. Related proteins such as SEC61 and ERAB are key associates in these pathways working together to facilitate the translocation and processing of nascent proteins entering the ER.

SPCS2 is linked with pancreatic diseases and certain liver dysfunctions due to its role in protein processing and secretion. Aberrant functioning of SPCS2 can disrupt normal protein folding and maturation potentially leading to conditions like pancreatitis. The protein's link to SEC11 in the protein processing pathways highlights shared dysfunction pathways that can influence disease severity and progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (PubMed : 34388369). Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site (By similarity).

See full target information SPCS2

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

American journal of men's health 18:15579883241297880 PubMed39558547

2024

SPC25 Activates the Warburg Effect to Inhibit Ferroptosis in Prostate Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mingqiang Su,Jingxian Luo,Wei Chen,Xianyong Li,Dayong Ye,Xiaofu Zeng,Guangqing Fu,Weiwei Xie,Yong Liang

Redox report : communications in free radical research 27:270-278 PubMed36357965

2022

Caffeine reduces oxidative stress to protect against hyperoxia-induced lung injury via the adenosine A2A receptor/cAMP/PKA/Src/ERK1/2/p38MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xijuan Wang,Shuai Lv,Jianwei Sun,Meihui Zhang,Lei Zhang,Yan Sun,Ziyan Zhao,Dandan Wang,Xinjing Zhao,Jiajie Zhang

Oncology reports 47: PubMed35293598

2022

SPC25 promotes hepatocellular carcinoma metastasis via activating the FAK/PI3K/AKT signaling pathway through ITGB4.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Kai Shi,Qiao-Li Shang,Yong-Fu Zhao

Cell death & disease 12:42 PubMed33414446

2021

TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Susu Guo,Yuxin Chen,Yueyue Yang,Xiao Zhang,Lifang Ma,Xiangfei Xue,Yongxia Qiao,Jiayi Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPCS2/SPC25 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB121395)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB121395)

Immunocytochemistry/ Immunofluorescence - Anti-SPCS2/SPC25 antibody (AB121395)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB121395)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB121395)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB121395)

Western blot - Anti-SPCS2/SPC25 antibody (AB121395)

Western blot - Anti-SPCS2/SPC25 antibody (AB121395)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise