AB242101

Anti-SPCS2/SPC25 antibody

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Rabbit Polyclonal SPCS2/SPC25 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human SPCS2 aa 150 to C-terminus.

대체 명칭 보기

KIAA0102, SPC25, SPCS2, Signal peptidase complex subunit 2, Microsomal signal peptidase 25 kDa subunit, SPase 25 kDa subunit

2 이미지

Supplier Data

Immunoprecipitation - Anti-SPCS2/SPC25 antibody (AB242101)

SPCS2/SPC25 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242101 at 20 μl/reaction. Western blot was performed from the immunoprecipitate using ab242101 at 1/250 dilution.

Lane 1 : ab242101 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-SPCS2/SPC25 antibody (ab242101)

Predicted band size: 25 kDa

false

Supplier Data

Western blot - Anti-SPCS2/SPC25 antibody (AB242101)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-SPCS2/SPC25 antibody (ab242101) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 25 kDa

false

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SPCS2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15005

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "20 μl/mg lysate", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to SPCS2/SPC25 immobilized on solid support.

보관 버퍼

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SPCS2 also called SPC25 is a component of the signal peptidase complex involved in cleaving signal peptides from nascent proteins during translocation into the endoplasmic reticulum. This protein has a molecular mass of approximately 27 kDa. SPCS2 is expressed in various tissues with notable levels in the liver pancreas and kidney. This broad expression suggests its importance across multiple physiological systems.

Biological function summary

The function of SPCS2/SPC25 relates to its role in the signal peptidase complex. It participates in the maturation of proteins by processing them for proper folding and export within the secretory pathway. As part of this multimeric complex in the endoplasmic reticulum membrane SPCS2 coordinates with other components like SEC11 to efficiently cleave signal peptides. Its function is critical for the regulation of protein synthesis and secretion processes.

Pathways

SPCS2 functions within the secretory pathways particularly in protein translocation into the endoplasmic reticulum. Its involvement in the ER-associated degradation pathway highlights its role in maintaining protein homeostasis. Related proteins such as SEC61 and ERAB are key associates in these pathways working together to facilitate the translocation and processing of nascent proteins entering the ER.

SPCS2 is linked with pancreatic diseases and certain liver dysfunctions due to its role in protein processing and secretion. Aberrant functioning of SPCS2 can disrupt normal protein folding and maturation potentially leading to conditions like pancreatitis. The protein's link to SEC11 in the protein processing pathways highlights shared dysfunction pathways that can influence disease severity and progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (PubMed : 34388369). Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site (By similarity).

See full target information SPCS2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPCS2/SPC25 antibody

Immunoprecipitation - Anti-SPCS2/SPC25 antibody (AB242101)

Western blot - Anti-SPCS2/SPC25 antibody (AB242101)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise