AB235598

Anti-SPCS3 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal SPCS3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPCS3 aa 1 to C-terminus.

대체 명칭 보기

SPC22, UNQ1841/PRO3567, SPCS3, Signal peptidase complex subunit 3, Microsomal signal peptidase 22/23 kDa subunit, SPC22/23, SPase 22/23 kDa subunit

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

Immunohistochemical analysis of ab235598 diluted at 1 : 150 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

Immunohistochemical analysis of ab235598 diluted at 1 : 150 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

Immunohistochemical analysis of ab235598 diluted at 1 : 150 and staining in paraffin-embedded mouse liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SPCS3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P61009

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SPCS3 also known as Signal Peptidase Complex Subunit 3 functions mechanically as a component of the signal peptidase complex which is responsible for cleaving the signal peptides from nascent proteins. It plays an important role in the protein maturation process within the endoplasmic reticulum. The protein is approximately 15 kDa in mass and is highly expressed in tissues such as the liver and pancreas. Its expression is critical in cells where active protein synthesis occurs.

Biological function summary

The signal peptidase complex including SPCS3 is involved in the processing and maturation of secretory and membrane proteins. SPCS3 forms part of a larger multisubunit complex that ensures proteins assume their proper functional forms. By cleaving signal peptides SPCS3 facilitates the proper localization and function of proteins within the cell. In doing so it contributes to cellular processes that depend on effective signal peptide processing such as growth and development.

Pathways

SPCS3 plays a significant role in the protein processing pathway in the endoplasmic reticulum. It interacts with other proteins involved in this pathway such as SPCS1 and SEC61 ensuring the correct translocation and maturation of newly synthesized polypeptides. The efficient functioning of SPCS3 in these pathways is important for maintaining cellular homeostasis and supporting protein synthesis and secretion.

Research links SPCS3 to conditions involving protein misfolding and secretion dysfunction such as cystic fibrosis and some forms of diabetes. In cystic fibrosis the malfunction of SPCS3 can lead to improper processing of the CFTR protein exacerbating disease symptoms. The protein's role in these diseases connects it to other factors involved in the pathology such as the CFTR protein. Understanding how SPCS3 influences these disorders can help develop targeted therapeutic strategies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Essential component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (PubMed : 27499293, PubMed : 34388369). Essential for the SPC catalytic activity, possibly by stabilizing and positioning the active center of the complex close to the lumenal surface (By similarity).. (Microbial infection) Plays an important role in virion production of flaviviruses such as West Nile virus, Japanese enchephalitis virus, Dengue virus type 2 and Yellow Fever virus.

See full target information SPCS3

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

BMC cancer 24:681 PubMed38834966

2024

PPIH acts as a potential predictive biomarker for patients with common solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ye,Jianchao Ying,Haixia Chen,Zhiping Wu,Chaolin Huang,Chuan Zhang,Zhitao Chen,Haini Chen

Nucleic acids research 52:4872-4888 PubMed38412296

2024

RNA-RNA interactions between respiratory syncytial virus and miR-26 and miR-27 are associated with regulation of cell cycle and antiviral immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Ressel,Sujai Kumar,Jose Roberto Bermúdez-Barrientos,Katrina Gordon,Julia Lane,Jin Wu,Cei Abreu-Goodger,Jürgen Schwarze,Amy H Buck

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPCS3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise