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AB244347

Anti-SPEN antibody

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Rabbit Polyclonal SPEN antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SPEN aa 100-300.

대체 명칭 보기

KIAA0929, MINT, SHARP, SPEN, Msx2-interacting protein, SMART/HDAC1-associated repressor protein, SPEN homolog

5 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPEN antibody (AB244347)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPEN antibody (AB244347)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for SPEN using ab244347 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPEN antibody (AB244347)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPEN antibody (AB244347)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for SPEN using ab244347 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPEN antibody (AB244347)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPEN antibody (AB244347)

Formalin-fixed, paraffin-embedded human skin tissue stained for SPEN using ab244347 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SPEN antibody (AB244347)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SPEN antibody (AB244347)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for SPEN (green) using ab244347 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPEN antibody (AB244347)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPEN antibody (AB244347)

Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for SPEN using ab244347 at 1/200 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SPEN aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

Q96T58

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보완 제품

Primary Antibodies

AB108937

Anti-Hes1 antibody [EPR4226]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] (AB108937)

  • 4
  • 15
Primary Antibodies

AB52627

Anti-Notch1 antibody [EP1238Y]

RabMAb|Recombinant|KO Validated|20ul selling size|Advanced Validation

Western blot - Anti-Notch1 antibody [EP1238Y] (AB52627)

  • 5
  • 18
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB23426

Anti-NOTCH3 antibody

BOND RX™ Validated

Western blot - Anti-NOTCH3 antibody (AB23426)

  • 4
  • 22
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SPEN also known as Split Ends or SHARP is a large nuclear protein with a mass of approximately 400 kDa. It is expressed in various tissues including the brain heart and lungs. Mechanically SPEN functions as an RNA-binding protein with C-terminal SPOC (Spen Paralog and Ortholog C-terminal) domains which allow it to interact with other cellular components. These interactions regulate transcription by modulating the activity of coactivators and corepressors at specific gene sites.
Biological function summary

SPEN plays a role in transcriptional repression and gene silencing by being part of a multiprotein complex. It interacts with nuclear receptors and RNA polymerase II inhibiting the transcriptional activity of target genes. SPEN helps regulate critical cellular processes such as differentiation and proliferation. It acts as a transcriptional corepressor by interacting with nucleic acids and influencing chromatin structure impacting gene expression in a context-dependent manner.

Pathways

SPEN holds an important role in the Notch signaling pathway. This pathway is important for cell fate determination and tissue homeostasis. SPEN also interacts with the estrogen receptor signaling pathway where it can influence the transcriptional outcome. Through these pathways SPEN associates with proteins such as RBPJ a transcriptional regulator in the Notch pathway and ERα (estrogen receptor alpha) a vital component in the estrogen signaling pathway.

SPEN has connections to certain cancers particularly breast cancer and neurological conditions like autism spectrum disorders. In breast cancer SPEN can interact with ERα influencing the proliferation of cancer cells through estrogen receptor signaling. In neurological conditions dysregulation of SPEN's interaction with proteins involved in neural development might contribute to disease mechanisms. Understanding these associations helps in potential therapeutic targeting of SPEN in relevant diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation : synergizes with RUNX2 to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA.
See full target information SPEN
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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