AB138511

Anti-SPINK5/LEKTI antibody [EPR8777]

RabMAb
Recombinant
각 태그의 뜻

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(4 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal SPINK5/LEKTI antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

대체 명칭 보기

Serine protease inhibitor Kazal-type 5, Lympho-epithelial Kazal-type-related inhibitor, LEKTI, SPINK5

2 이미지

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Western blot - Anti-SPINK5/LEKTI antibody [EPR8777] (AB138511)

All lanes:

Western blot - Anti-SPINK5/LEKTI antibody [EPR8777] (ab138511) at 1/1000 dilution

Lane 1:

Fetal heart cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 121 kDa

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Unknown

Western blot - Anti-SPINK5/LEKTI antibody [EPR8777] (AB138511)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SPINK5/LEKTI antibody [EPR8777] (ab138511) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Mouse heart lysates at 15 µg

Lane 3:

Rat heart lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 121 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8777

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SPINK5 also known as LEKTI (lympho-epithelial Kazal-type-related inhibitor) is a protease inhibitor with a molecular mass of approximately 130 kDa. This protein is predominantly expressed in epithelial tissues such as the skin thymus and mucosal surfaces like the lung and gut. SPINK5/LEKTI plays a mechanical role in inhibiting serine proteases which are critical for skin barrier functions. It regulates the activity of enzymes that are involved in the process of desquamation and maintaining skin integrity.

Biological function summary

SPINK5/LEKTI functions to maintain the balance of protease activity within skin tissue. It is part of a larger complex that regulates barrier homeostasis and influences desquamation by inhibiting enzymes such as kallikreins. This protease inhibition reduces excessive protease activity therefore preserving the epidermal barrier. By controlling the breakdown of structural components the protein ensures proper skin resilience and hydration retention.

Pathways

SPINK5/LEKTI is involved in the epidermal differentiation complex and interacts within the kallikrein-related pathway. It plays a role in regulating the kallikrein protease family including proteins such as KLK5 and KLK7 which are important for skin shedding and barrier formation. The inhibition of these proteases ensures a balanced turnover of skin cells preventing unwanted degradation of the skin's protective layer. SPINK5/LEKTI participates in pathways that balance cell renewal and contribute to protective mechanisms against environmental damage.

SPINK5/LEKTI is most notably linked to Netherton syndrome a genetic disorder characterized by compromised skin barrier function chronic inflammation and allergy susceptibility. Mutations in SPINK5 lead to uncontrolled protease activity due to reduced inhibition predominantly impacting related kallikrein activities. This connection extends to disorders of skin barrier failure. Additionally SPINK5/LEKTI may play a role in atopic dermatitis where aberrant protease activity contributes to the pathophysiology through similar mechanisms involving proteins like FLG (filaggrin) which affect skin hydration and barrier function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin.

See full target information SPINK5

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Acta biochimica et biophysica Sinica : PubMed40999902

2025

KDM4A regulates microglial polarization after ischemic stroke by regulating SPINK5 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Min,Lei Xian,Ting Liu,Mengze Wang,Qing Zhao,Jiayi Hu,Rui Jing

Cellular & molecular biology letters 27:48 PubMed35705923

2022

Metformin inhibits melanoma cell metastasis by suppressing the miR-5100/SPINK5/STAT3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Suwei,Xiao Yanbin,Wang Jianqiang,Ma Xiang,Peng Zhuohui,Kang Jianping,Wang Yunqing,Li Zhen

Experimental dermatology 31:223-232 PubMed34378233

2021

STAT3 maintains skin barrier integrity by modulating SPINK5 and KLK5 expression in keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jaehyung Kim,Min-Gyu Kim,Sang Hoon Jeong,Hee Joo Kim,Sang Wook Son

Journal of leukocyte biology 103:799-805 PubMed29537705

2018

Frontline Science: Microbiota reconstitution restores intestinal integrity after cisplatin therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Alfredo Perales-Puchalt,Jairo Perez-Sanz,Kyle K Payne,Nikolaos Svoronos,Michael J Allegrezza,Ricardo A Chaurio,Carmen Anadon,Joseph Calmette,Subir Biswas,Jessica A Mine,Tara Lee Costich,Logan Nickels,Jayamanna Wickramasinghe,Melanie R Rutkowski,Jose R Conejo-Garcia

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPINK5/LEKTI antibody [EPR8777]

Western blot - Anti-SPINK5/LEKTI antibody [EPR8777] (AB138511)

Western blot - Anti-SPINK5/LEKTI antibody [EPR8777] (AB138511)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise