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AB122188

Anti-SPOCD1 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal SPOCD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPOCD1 aa 950-1100.

대체 명칭 보기

SPOC domain-containing protein 1, SPOCD1

6 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (AB122188)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (AB122188)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-SPOCD1 antibody (AB122188)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SPOCD1 antibody (AB122188)

Immunocytochemistry/immunofluorescence analysis of A431 cells labelling SPOCD1 with ab122188 shows positive staining in the nucleus and nucleoli.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (AB122188)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (AB122188)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (AB122188)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (AB122188)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (AB122188)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (AB122188)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-SPOCD1 antibody (AB122188)
  • WB

Supplier Data

Western blot - Anti-SPOCD1 antibody (AB122188)

All lanes:

Western blot - Anti-SPOCD1 antibody (ab122188) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate

Lane 3:

Human plasma (IgG/HSA depleted) lysate

Predicted band size: 130 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SPOCD1 aa 950-1100. The exact immunogen used to generate this antibody is proprietary information.

Q6ZMY3

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보완 제품

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  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SPOCD1 also known as SPOC domain-containing protein 1 is a protein with a molecular mass of approximately 105 kDa. This protein plays an important mechanical role in transcriptional regulation by influencing the chromatin state. SPOCD1 appears in the nucleus of cells and exhibits expression in tissues including the testes and adrenal glands. Its involvement is significant in processes requiring DNA-templated regulation.
Biological function summary

SPOCD1 acts as a part of large transcriptional complexes influencing gene expression through modification of chromatin structure. It interacts with other chromatin-associated proteins which assists in promoting or repressing transcription depending on the required cellular context. These interactions suggest SPOCD1's role as a mediator connecting transcription factors to chromatin remodeling machinery impacting gene activity directly.

Pathways

SPOCD1 is involved in pathways such as the regulatory pathway for RNA polymerase II-mediated transcription. It works closely with proteins like HDAC1 and components of the SWI/SNF complex that facilitate chromatin remodeling. These pathways highlight its role in modulating the access of transcription machinery to DNA highlighting its importance in maintaining normal transcriptional flow within cells.

SPOCD1 exhibits connections to cancer and infertility. Altered expression or mutations in SPOCD1 have links to tumorigenesis where the deregulated control of gene transcription may lead to uncontrolled cell proliferation. Moreover in the context of infertility SPOCD1 plays a role in germ cell development and its dysregulation can impact gametogenesis. These associations also involve proteins like BRD4 and DDX4 which work in concert with SPOCD1 to help maintain cellular and developmental processes.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Protein adapter that acts as an essential executor of PIWIL4-piRNA pathway directed transposon DNA methylation and silencing in the male embryonic germ cells (PubMed : 38359823). Recruited to young transposons, which are specifically marked with histone H3 trimethylated at both 'Lys-4' and 'Lys-9' (H3K4me3K9me3), via its association with SPIN1 chromatin reader, and associates with the de novo DNA methylation machinery and repressive chromatin remodeling complexes (By similarity). Following this, PIWIL4 engages with nascent transposable element transcript to direct piRNA-directed DNA methylation. Not required for piRNA biosynthesis (By similarity).
See full target information SPOCD1
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of translational medicine 23:902 PubMed40804742

2025

Cancer cell SPOCD1 promotes colorectal cancer liver metastasis by activating the CXCL12/CXCR4 signaling pathway in cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Geng Peng,Lin Zhong,Lina Luo,Yongle Ju,Yan Lu,Lui Ng,Dominic Chi-Chung Foo,Fu Cheng,Luji Huang,Manzhao Ouyang

Molecular medicine reports 17:3218-3225 PubMed29257309

2017

SPOCD1 promotes cell proliferation and inhibits cell apoptosis in human osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinqian Liang,Hong Zhao,Jianhua Hu,Yong Liu,Zheng Li

Journal of cellular biochemistry 119:1841-1854 PubMed28815726

2017

Differential expression profile analysis of lncRNA UCA1α regulated mRNAs in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Hong Zhang,Xu Li,Wei Chen
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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