Anti-sRANKL antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sRANKL antibody (AB62516)

Immunohistochemical analysis of paraffin-embedded human liver tissue using ab62516 at 5μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4°C. Goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as a secondary. Counter stained with Hematoxylin.

• WB

Supplier Data

Western blot - Anti-sRANKL antibody (AB62516)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-sRANKL antibody (ab62516) at 1 µg/mL

Lane 1:

A431 cell lysate at 15 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 35 kDa

Observed band size: 36 kDa

false

• WB

Unknown

Western blot - Anti-sRANKL antibody (AB62516)

Blocking buffer : 5% NFDM/TBST.

Lane 1:

Western blot - Anti-sRANKL antibody (ab62516) at 0.25 µg/mL

Lane 2:

Western blot - Anti-sRANKL antibody (ab62516) at 0.5 µg/mL

All lanes:

Rat liver tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 35 kDa

Observed band size: 30 kDa

false

• WB

Supplier Data

Western blot - Anti-sRANKL antibody (AB62516)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-sRANKL antibody (ab62516) at 2 µg/mL

Lane 1:

Mouse kidney tissue lysate at 15 µg

Lane 2:

Mouse skin tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 35 kDa

Observed band size: 36 kDa

false