AB30682

Anti-SREBP2 antibody

Name: Anti-SREBP2 항체 (ab30682) | Abcam
Brand: Abcam Limited
SKU: AB30682
Rating: 4 (12 reviews)

Anti-SREBP2 항체

(12 리뷰들)

(214 제품이 사용된 논문 )

Anti-SREBP2 antibody (ab30682) is a rabbit polyclonal antibody detecting SREBP2 in Western Blot, IHC-Fr, ICC/IF. Suitable for Human, Rat.

- Over 160 publications
- Trusted since 2006

대체 명칭 보기

BHLHD2, SREBP2, SREBF2, Sterol regulatory element-binding protein 2, SREBP-2, Class D basic helix-loop-helix protein 2, Sterol regulatory element-binding transcription factor 2, bHLHd2

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB30682)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB30682)

ICC/IF image of ab30682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30682, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

AbReview35456****

Western blot - Anti-SREBP2 antibody (AB30682)

Blocking : 7% milk for 60 minutes at 20^oC.

All lanes:

Western blot - Anti-SREBP2 antibody (ab30682) at 1/1000 dilution

All lanes:

Human retina whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit polyclonal at 1/1000 dilution

Predicted band size: 123 kDa

Observed band size: 126 kDa,55 kDa,60 kDa

true

Exposure time: 6min

This image is courtesy of an Abreview by Dongil Kim.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, IHC-Fr, WB

applications

Immunogen

Synthetic Peptide within Human SREBF2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q12772

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "Block with 5% non-fat dry milk in TBS pH 7.4 for 1-2 hours at room temperature or over night at 4°C.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "Block with 5% non-fat dry milk in TBS pH 7.4 for 1-2 hours at room temperature or over night at 4°C.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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제품 세부 정보

Product Specifications
Anti-SREBP2 antibody (ab30682) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-Fr, WB in chicken, human, rat samples.
Anti-SREBP2 antibody (ab30682) specifically detects SREBP2 (UniProt ID: Q12772; Molecular weight: 124kDa) and is sold in 250 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-SREBP2 antibody (ab30682) has high sensitivity and specificity.
Anti-SREBP2 antibody (ab30682) has been cited over 165 times in peer reviewed journals and is trusted by the scientific community.
Anti-SREBP2 antibody (ab30682) has 11 independent reviews from customers.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine), 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Sterol regulatory element-binding protein 2. Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane (PubMed : 32322062). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis (PubMed : 32322062).. Processed sterol regulatory element-binding protein 2. Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis (PubMed : 12177166, PubMed : 32322062). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') (PubMed : 12177166, PubMed : 7903453). Regulates transcription of genes related to cholesterol synthesis pathway (PubMed : 12177166, PubMed : 32322062).

See full target information SREBF2

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제품이 사용된 논문 (214)

Recent publications for all applications. Explore the 전체 목록 and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e71051 PubMed40955587

2025

NONO Maintains SREBP-Regulated Cholesterol Biosynthesis via RNA Binding in Neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Song Zhang,Hayley Ingram,Jack Cooper,Alina Naveed,Stefan G Kathman,Garrett L Lindsey,Tao Liu,Charles S Bond,Jamie I Fletcher,Benjamin F Cravatt,Archa H Fox

Scientific reports 15:23103 PubMed40595126

2025

SREBP-2 promotes cancer progression through the mevalonate-Akt pathway in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie You,Lili Su,Shuping Weng,Jing Li,Xingguang Wang,Bin Liang,Daowei Li,Lijun Li,Haiquan Chen

Cancer communications (London, England) 45:1038-1071 PubMed40443016

2025

Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghan Zhu,Yixiao Xiong,Yuxin Zhang,Huifang Liang,Kun Cheng,Yuanxiang Lu,Guangzhen Cai,Yang Wu,Yunhui Fan,Xiaoping Chen,Hong Zhu,Zeyang Ding,Wanguang Zhang

European journal of medical research 30:296 PubMed40247356

2025

Mammalian Ste20-like kinase 1 regulates AMPK to mitigate the progression of non-alcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Wang,Chenglei Zhang,Jie Ma,Jiarui Li,Yuanyuan Wu,Yanru Ren,Jianning Li,Yan Li,Yi Yang

Cell death & disease 16:308 PubMed40240362

2025

IGF2BP3/ESM1/KLF10/BECN1 positive feedback loop: a novel therapeutic target in ovarian cancer via lipid metabolism reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Anbo Gao,Juan Zou,Tian Zeng,Mei Qin,Xing Tang,Ting Yi,Guangming Song,Jie Zhong,Yuhuan Zeng,Wenchao Zhou,Qin Gao,Qunfeng Zhang,Juan Zhang,Yukun Li

International journal of chronic obstructive pulmonary disease 20:411-423 PubMed40008109

2025

Cholesterol Accumulation Enhances Cigarette Smoke-Induced Airway Epithelial Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Du Jing,Jin-Kang Yu,Hai-Pin Chen,Ling-Ling Dong,Wen Li,Zhou-Yang Li,Jie-Sen Zhou

The Journal of clinical investigation 135: PubMed39998898

2025

An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangioma.

Applications

Unspecified application

Species

Unspecified reactive species

Annegret Holm,Matthew S Graus,Jill Wylie-Sears,Jerry Wei Heng Tan,Maya Alvarez-Harmon,Luke Borgelt,Sana Nasim,Long Chung,Ashish Jain,Mingwei Sun,Liang Sun,Pascal Brouillard,Ramrada Lekwuttikarn,Yanfei Qi,Joyce Teng,Miikka Vikkula,Harry Kozakewich,John B Mulliken,Mathias Francois,Joyce Bischoff

Nature communications 16:1241 PubMed39890808

2025

Copy number amplification of FLAD1 promotes the progression of triple-negative breast cancer through lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Qing Song,Tian-Jian Yu,Yang Ou-Yang,Jia-Han Ding,Yi-Zhou Jiang,Zhi-Ming Shao,Yi Xiao

Experimental and therapeutic medicine 29:46 PubMed39885913

2025

Lindl. alkaloids improve lipid metabolism by increasing LDL uptake through regulation of the LXRα/IDOL/LDLR pathway and inhibition of PCSK9 expression in HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Sun,Hao-Rui Liu,Ya-Xin Zhu,Wei Zhang,Jing-Shan Shi,Qin Wu,Rui-Xia Xu

Cancer cell international 25:25 PubMed39871246

2025

DLAT is involved in ovarian cancer progression by modulating lipid metabolism through the JAK2/STAT5A/SREBP1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Shen Luo,Yue Yin,Yang Liu,Xiaomei Sun,Ling Qiu,Xin Wu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SREBP2 antibody

Anti-SREBP2 항체

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB30682)

Western blot - Anti-SREBP2 antibody (AB30682)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (214)

Product promise