AB121977

Anti-srGAP2 antibody

Name: Anti-srGAP2 antibody
Brand: Abcam Limited
SKU: AB121977
Rating: 4 (2 reviews)

(2 리뷰들)

(4 제품이 사용된 논문 )

Rabbit Polyclonal srGAP2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, African green monkey samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SRGAP2 aa 950 to C-terminus.

대체 명칭 보기

ARHGAP34, FNBP2, KIAA0456, SRGAP2A, SRGAP2, SLIT-ROBO Rho GTPase-activating protein 2, srGAP2, Formin-binding protein 2, Rho GTPase-activating protein 34

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)

Immunohistochemical analysis of human testis tissue labelling SRGAP2 using ab121977 at 1/50 dilution. Positive nuclear and cytoplasmic staining observed in Leydig cells and seminiferous duct cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)

Immunohistochemical analysis of human kidney tissue labelling SRGAP2 using ab121977 at 1/50 dilution. Positive nuclear and cytoplasmic staining observed in distal tube cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)

Immunohistochemical analysis of human bone marrow labelling SRGAP2 using ab121977 at 1/50 dilution. Positive nuclear and cytoplasmic staining observed in hematopoietic cells.

Immunocytochemistry/ Immunofluorescence - Anti-srGAP2 antibody (AB121977)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-srGAP2 antibody (AB121977)

Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121977 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Collaborator

Western blot - Anti-srGAP2 antibody (AB121977)

Blocking : 5% MIlk

All lanes:

Western blot - Anti-srGAP2 antibody (ab121977) at 1/100 dilution

Lane 1:

COS7 Whole cell lysate at 30 µg

Lane 2:

HEK293H Whole Cell Lysate at 30 µg

Lane 3:

HEK293H Over expressing human srGAP1 at 30 µg

Lane 4:

HEK293H Over expressing human srGAP2 at 30 µg

Lane 5:

HEK293H Over expressing human srGAP3 at 30 µg

Lane 6:

HeLa cells over expressing mouse srGAP2 at 30 µg

Lane 7:

Mouse E14 hindbrain lysate at 30 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilution

Predicted band size: 121 kDa

Observed band size: 170 kDa,200 kDa

true

Exposure time: 5min

This image is courtesy of Alain Chedotal (Institut Vision, France)

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, African green monkey

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SRGAP2 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75044

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/20", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/20", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab121977 is mono-specific.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Postsynaptic RAC1 GTPase activating protein (GAP) that plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex (PubMed : 20810653, PubMed : 27373832, PubMed : 28333212). Regulates excitatory and inhibitory synapse maturation and density in cortical pyramidal neurons (PubMed : 22559944, PubMed : 27373832). SRGAP2/SRGAP2A limits excitatory and inhibitory synapse density through its RAC1-specific GTPase activating activity, while it promotes maturation of both excitatory and inhibitory synapses through its ability to bind to the postsynaptic scaffolding protein HOMER1 at excitatory synapses, and the postsynaptic protein GPHN at inhibitory synapses (By similarity). Mechanistically, acts by binding and deforming membranes, thereby regulating actin dynamics to regulate cell migration and differentiation (PubMed : 27373832). Promotes cell repulsion and contact inhibition of locomotion : localizes to protrusions with curved edges and controls the duration of RAC1 activity in contact protrusions (By similarity). In non-neuronal cells, may also play a role in cell migration by regulating the formation of lamellipodia and filopodia (PubMed : 20810653, PubMed : 21148482).

See full target information SRGAP2

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Genes 16: PubMed40282329

2025

Techniques for Validating CRISPR Changes Using RNA-Sequencing Data.

Applications

Unspecified application

Species

Unspecified reactive species

Susan K Rathe,Tracy A Marko,Elizabeth N Edwards,Paige Hazelton Ridder,Jyotika Varshney,Kyle B Williams,James E Johnson,Branden S Moriarity,David A Largaespada

BMC nephrology 23:151 PubMed35436879

2022

Impact of klotho on the expression of SRGAP2a in podocytes in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Donghua Jin,Miao Jia,Yuxian Xie,Lihua Lin,Hong Qiu,Guoyuan Lu

Diabetes 67:717-730 PubMed29242313

2017

Dissection of Glomerular Transcriptional Profile in Patients With Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Pan,Song Jiang,Qing Hou,Dandan Qiu,Jingsong Shi,Ling Wang,Zhaohong Chen,Mingchao Zhang,Aiping Duan,Weisong Qin,Ke Zen,Zhihong Liu

Scientific reports 6:39059 PubMed27966608

2016

Slit-Robo GTPase-Activating Protein 2 as a metastasis suppressor in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tracy A Marko,Ghaidan A Shamsan,Elizabeth N Edwards,Paige E Hazelton,Susan K Rathe,Ingrid Cornax,Paula R Overn,Jyotika Varshney,Brandon J Diessner,Branden S Moriarity,M Gerard O'Sullivan,David J Odde,David A Largaespada

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-srGAP2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)

Immunocytochemistry/ Immunofluorescence - Anti-srGAP2 antibody (AB121977)

Western blot - Anti-srGAP2 antibody (AB121977)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise