AB200199

Anti-SRP72 antibody [EPR17143]

RabMAb
Recombinant
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal SRP72 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

대체 명칭 보기

Signal recognition particle subunit SRP72, SRP72, Signal recognition particle 72 kDa protein

4 이미지

Flow Cytometry (Intracellular) - Anti-SRP72 antibody [EPR17143] (AB200199)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SRP72 antibody [EPR17143] (AB200199)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling SRP72 with ab200199 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-SRP72 antibody [EPR17143] (AB200199)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SRP72 antibody [EPR17143] (ab200199) at 1/5000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-SRP72 antibody [EPR17143] (AB200199)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SRP72 antibody [EPR17143] (ab200199) at 1/5000 dilution

All lanes:

Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-SRP72 antibody [EPR17143] (AB200199)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SRP72 antibody [EPR17143] (ab200199) at 1/1000 dilution

All lanes:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 75 kDa,89 kDa

Observed band size: 75 kDa,89 kDa

false

Exposure time: 1min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17143

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER) (PubMed : 34020957). The SRP complex interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the ER (PubMed : 34020957). The SRP complex targets the ribosome-nascent chain complex to the SRP receptor (SR), which is anchored in the ER, where SR compaction and GTPase rearrangement drive cotranslational protein translocation into the ER (PubMed : 34020957). Binds the signal recognition particle RNA (7SL RNA) in presence of SRP68 (PubMed : 21073748, PubMed : 27899666). Can bind 7SL RNA with low affinity (PubMed : 21073748, PubMed : 27899666). The SRP complex possibly participates in the elongation arrest function (By similarity).

See full target information SRP72

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PLoS pathogens 20:e1012766 PubMed39621795

2024

Flaviviruses induce ER-specific remodelling of protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ho Him Wong,Dorian Richard Kenneth Crudgington,Lewis Siu,Sumana Sanyal

International journal of molecular sciences 22: PubMed34066088

2021

Alternative C3 Complement System: Lipids and Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Maisa Garcia-Arguinzonis,Elisa Diaz-Riera,Esther Peña,Rafael Escate,Oriol Juan-Babot,Pedro Mata,Lina Badimon,Teresa Padro

Molecular cell 75:1103-1116.e9 PubMed31420216

2019

Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.

Applications

Unspecified application

Species

Unspecified reactive species

Dianrong Li,Jie Chen,Youwei Ai,Xiaoqiong Gu,Li Li,Di Che,Zhaodi Jiang,Lin Li,She Chen,Huangwei Huang,Jiawen Wang,Tao Cai,Yang Cao,Xiangbin Qi,Xiaodong Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SRP72 antibody [EPR17143]

Flow Cytometry (Intracellular) - Anti-SRP72 antibody [EPR17143] (AB200199)

Western blot - Anti-SRP72 antibody [EPR17143] (AB200199)

Western blot - Anti-SRP72 antibody [EPR17143] (AB200199)

Western blot - Anti-SRP72 antibody [EPR17143] (AB200199)

관련 conjugated 항체와 다양한 조성의 항체 (8)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise