Anti-SRRM1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SRRM1 antibody (AB221061)

Immunocytochemical analysis of HEK-293 (Human epithelial cell line from embryonic kidney) whole cells labeling SRRM1 (green) in the nuclear speckles with ab221061 at 2 μg/mL.

• WB

CiteAb

Western blot - Anti-SRRM1 antibody (AB221061)

SRRM1 western blot using anti-SRRM1 antibody ab221061. Publication image and figure legend from Ilik, İ. A., Malszycki, M., et al., 2020, Elife, PubMed 33095160.

ab221061 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab221061 please see the product overview.

Co-depletion of SON and SRRM2 in SRRM2tr0+GFP HEK293 cells leads to loss of NS.(A) RBM25 IF signal is shown for four individual cells in each siRNA treatment (control, SRRM2, SON or SRRM2, and SON siRNA) in SRRM2tr0+GFP HEK293 cells. RBM25 signal diffuses out of NS upon SON depletion but is completely lost only in the double knock-down cells. (B) SRRM1 IF signal is shown for four individual cells in each siRNA treatment (control, SRRM2, SON or SRRM2 and SON siRNA) in SRRM2tr0+GFP HEK293 cells. SRRM1 signal diffuses out of NS upon SRRM2 depletion and is mostly diffused in the nucleus in the double knock-down cells. (C) PNN IF signal is shown for four individual cells in each siRNA treatment (control, SRRM2, SON or SRRM2 and SON siRNA) in SRRM2tr0+GFP HEK293 cells. PNN signal diffuses out of NS upon SON depletion and is mostly diffused in the nucleus in the double knock-down cells. Scale bars = 5 µm (D) Distribution plots showing the ratio of signal detected in NS over signal detected in the nucleus of each cell, in each condition. The dashed line indicates the median ratio in each condition, similar to Figure 5C (for RBM25 staining in control siRNA n = 321, in SON-KD n = 187; in SRRM2-KD n = 249, in double-KD n = 202; for SRRM1 staining in control siRNA n = 155, in SON-KD n = 150; in SRRM2-KD n = 197, in double-KD n = 262; for PNN staining in control siRNA n = 204, in SON-KD n = 264; in SRRM2-KD n = 299, in double-KD n = 229). The double knock-down of SRRM2 and SON leads to the most significant loss of signal localised to the NS for RBM25, SRRM1 and PNN. (E) The diffusion of RBM25, SRRM1 or PNN out of the NS is not caused by the down-regulation of the protein levels as shown by western blot (see Lanes 1 and 4).Figure 5—figure supplement 4—source data 1.Contains ilastik models (.ilp) used to train for nuclear speckles, and Cell Profiler pipelines (.cpproj) used to process the probability maps generated by ilastik.The outputs from Cell Profiler (.csv) files are used to generate the figures in the Jupyter Lab environment (.ipynb), shown in Figure 5—figure supplement 4.Contains ilastik models (.ilp) used to train for nuclear speckles, and Cell Profiler pipelines (.cpproj) used to process the probability maps generated by ilastik.The outputs from Cell Profiler (.csv) files are used to generate the figures in the Jupyter Lab environment (.ipynb), shown in Figure 5—figure supplement 4.

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