AB224053

Anti-SSBP1 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal SSBP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SSBP1 aa 1 to C-terminus.

대체 명칭 보기

SSBP, SSBP1, Mt-SSB, MtSSB, PWP1-interacting protein 17

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB224053)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB224053)

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human duodenum sections labeling SSBP1 with ab224053 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB224053)

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human cerebral cortex sections labeling SSBP1 with ab224053 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB224053)

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human testis sections labeling SSBP1 with ab224053 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (AB224053)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (AB224053)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SSBP1 (green) using ab224053 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB224053)

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human pancreas sections labeling SSBP1 with ab224053 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-SSBP1 antibody (AB224053)

All lanes:

Western blot - Anti-SSBP1 antibody (ab224053) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 17 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SSBP1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q04837

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SSBP1 also known as Single-Stranded DNA Binding Protein 1 is a mitochondrial protein with an approximate mass of 14 kDa. SSBP1 binds to single-stranded DNA and stabilizes it during DNA replication and repair processes. It functions by preventing the DNA from re-annealing or forming secondary structures which helps in maintaining genomic stability. This protein is mostly expressed in tissues with high energy demands such as muscle and liver reflecting its significant role in mitochondrial activities.

Biological function summary

Single-Stranded DNA Binding Protein 1 participates in essential mitochondrial DNA maintenance processes and interacts with other proteins to form functional complexes. It associates with mitochondrial DNA polymerase gamma and other replication machinery components to ensure accurate DNA replication. These interactions are significant for proper mitochondrial function since they prevent mutations that could compromise cellular energy production.

Pathways

SSBP1 engages actively in the mitochondrial DNA replication and repair pathway. It works closely with proteins like TWINKLE helicase and DNA polymerase gamma complex facilitating efficient replication and repair of mitochondrial DNA. These pathways are pivotal for mitochondrial biogenesis and function impacting cellular energy metabolism and overall mitochondrial health.

Alterations in SSBP1 have links to mitochondrial dysfunctions including disorders like mitochondrial myopathy and progressive external ophthalmoplegia. Dysfunctional SSBP1 may lead to impaired DNA replication or repair in mitochondria contributing to these conditions. Additionally it relates to TWINKLE helicase mutations which also disrupt mitochondrial DNA maintenance further emphasizing the importance of SSBP1 in maintaining cellular energy balance.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA) (PubMed : 21953457, PubMed : 23290262, PubMed : 31550240). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork (PubMed : 12975372, PubMed : 15167897, PubMed : 21953457, PubMed : 26446790, PubMed : 31550240). Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity (PubMed : 21953457, PubMed : 26446790, PubMed : 31550240). In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK (PubMed : 12975372). May also function in mtDNA repair (PubMed : 23290262).

See full target information SSBP1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death discovery 11:400 PubMed40849295

2025

DCTPP1 regulates oxidative stress homeostasis via AUF1 in human villous trophoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yajuan Lu,Xue Wu,Lei He,Peng Pan,Anqi Zhao,Tangli Kan,Yuting Chu,Jinglin Dong,Shuangkai Xu,Xiaofang Tan,Xiaoqing Yang

Translational cancer research 14:112-128 PubMed39974423

2025

Mitochondrial single-stranded DNA binding protein 1 (SSBP1) high expression as a potential biomarker and association with poor prognosis in hepatocellular carcinoma (HCC).

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ru Lin,Jian-Kang Li,Xi Yang,Bao-Ru Dong,Ru-Ai Liu,Xin-Meng Wang,Min Yu,Wei Xiong

Redox biology 52:102303 PubMed35390676

2022

SSBP1 drives high fructose-induced glomerular podocyte ferroptosis via activating DNA-PK/p53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Yuan Wu,Zi-Xuan Wang,Tu-Shuai Li,Xiao-Qin Ding,Zhi-Hong Liu,Jie Yang,Lei Fang,Ling-Dong Kong

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SSBP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB224053)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB224053)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB224053)

Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (AB224053)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB224053)

Western blot - Anti-SSBP1 antibody (AB224053)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise