AB74710

Anti-SSBP1 antibody

Name: Anti-SSBP1 antibody
Brand: Abcam Limited
SKU: AB74710
Rating: 3 (1 reviews)

(1 리뷰)

(3 제품이 사용된 논문 )

Rabbit Polyclonal SSBP1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SSBP1.

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (AB74710)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (AB74710)

Immunofluorescence analysis of A549 cells, using ab74710 at a dilution of 1/500. Left-hand panel : no peptide; right-hand panel : with peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB74710)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB74710)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using ab74710 at a dilution of 1/50. Left-hand panel : no peptide; right-hand panel : with peptide.

Unknown

Western blot - Anti-SSBP1 antibody (AB74710)

Secondary antibody - anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-SSBP1 antibody (ab74710) at 1/500 dilution

Lane 1:

Extracts from HeLa cells at 5 µg with no peptide

Lane 2:

Extracts from HUVEC cells at 5 µg with no peptide

Lane 3:

Extracts from HUVEC cells at 5 µg with peptide

Predicted band size: 17 kDa

Observed band size: 17 kDa,26 kDa,45 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human SSBP1. The exact immunogen used to generate this antibody is proprietary information.

Q04837

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SSBP1 also known as Single-Stranded DNA Binding Protein 1 is a mitochondrial protein with an approximate mass of 14 kDa. SSBP1 binds to single-stranded DNA and stabilizes it during DNA replication and repair processes. It functions by preventing the DNA from re-annealing or forming secondary structures which helps in maintaining genomic stability. This protein is mostly expressed in tissues with high energy demands such as muscle and liver reflecting its significant role in mitochondrial activities.

Biological function summary

Single-Stranded DNA Binding Protein 1 participates in essential mitochondrial DNA maintenance processes and interacts with other proteins to form functional complexes. It associates with mitochondrial DNA polymerase gamma and other replication machinery components to ensure accurate DNA replication. These interactions are significant for proper mitochondrial function since they prevent mutations that could compromise cellular energy production.

Pathways

SSBP1 engages actively in the mitochondrial DNA replication and repair pathway. It works closely with proteins like TWINKLE helicase and DNA polymerase gamma complex facilitating efficient replication and repair of mitochondrial DNA. These pathways are pivotal for mitochondrial biogenesis and function impacting cellular energy metabolism and overall mitochondrial health.

Alterations in SSBP1 have links to mitochondrial dysfunctions including disorders like mitochondrial myopathy and progressive external ophthalmoplegia. Dysfunctional SSBP1 may lead to impaired DNA replication or repair in mitochondria contributing to these conditions. Additionally it relates to TWINKLE helicase mutations which also disrupt mitochondrial DNA maintenance further emphasizing the importance of SSBP1 in maintaining cellular energy balance.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA) (PubMed : 21953457, PubMed : 23290262, PubMed : 31550240). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork (PubMed : 12975372, PubMed : 15167897, PubMed : 21953457, PubMed : 26446790, PubMed : 31550240). Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity (PubMed : 21953457, PubMed : 26446790, PubMed : 31550240). In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK (PubMed : 12975372). May also function in mtDNA repair (PubMed : 23290262).

See full target information SSBP1

대체 명칭 보기

SSBP, SSBP1, Mt-SSB, MtSSB, PWP1-interacting protein 17

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in genetics 10:1393 PubMed32082360

2020

RNase H1 Regulates Mitochondrial Transcription and Translation the Degradation of 7S RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Aurelio Reyes,Joanna Rusecka,Katarzyna Tońska,Massimo Zeviani

Oncotarget 8:110861-110876 PubMed29340022

2017

A functional polymorphism of gene predicts prognosis and response to chemotherapy in resected gastric cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuchen Li,Falin Qu,Renli Li,Xianli He,Yulong Zhai,Weigang Chen,Yong Zheng

DNA repair 12:177-87 PubMed23290262

2013

Alkyladenine DNA glycosylase (AAG) localizes to mitochondria and interacts with mitochondrial single-stranded binding protein (mtSSB).

Applications

Unspecified application

Species

Unspecified reactive species

Barbara van Loon,Leona D Samson

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SSBP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (AB74710)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB74710)

Western blot - Anti-SSBP1 antibody (AB74710)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise