Anti-SSTR4 antibody [EPR29682-48]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (AB324103)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling SSTR4 with ab324103 at 1/100 (5.14 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human lung.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (AB324103)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SSTR4 with ab324103 at 1/100 (5.14 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human colon.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (AB324103)

Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling SSTR4 with ab324103 at 1/100 (5.14 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human astrocytoma.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (AB324103)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SSTR4 with ab324103 at 1/100 (5.14 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human cerebrum.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

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Western blot - Anti-SSTR4 antibody [EPR29682-48] (AB324103)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : colon, liver, lung, testis.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10804219, PMID : 34884783).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SSTR4 antibody [EPR29682-48] (ab324103) at 1/1000 dilution

Lane 1:

Human striatum tissue lysate at 48 µg

Lane 2:

Human colon tissue lysate at 48 µg

Lane 3:

Human liver tissue lysate at 48 µg

Lane 4:

Human thalamus tissue lysate at 48 µg

Lane 5:

Human lung tissue lysate at 48 µg

Lane 6:

Human testis tissue lysate at 48 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 42-50 kDa,36 kDa

false

Exposure time: 136s

• I-ELISA

Supplier Data

Indirect ELISA - Anti-SSTR4 antibody [EPR29682-48] (AB324103)

Indirect ELISA analysis of ab324103 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human SSTR4, Human SSTR1.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with human SSTR1.