AB236415

Anti-SSX2 antibody [OTI4A11]

Be the first to review this product! 리뷰 제출

(1 출판물)

Mouse Monoclonal SSX2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SSX2.

대체 명칭 보기

SSX2A, SSX2B, SSX2, Protein SSX2, Cancer/testis antigen 5.2, Tumor antigen HOM-MEL-40, CT5.2

10 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human colon adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human endometrium tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human bladder carcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human colon tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human ovary tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human ovary adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human breast adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human prostate tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SSX2 antibody [OTI4A11] (AB236415)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SSX2 antibody [OTI4A11] (AB236415)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY SSX2 stained for SSX2 (green) using ab236415 at 1/100 dilution in ICC/IF.

Supplier Data

Western blot - Anti-SSX2 antibody [OTI4A11] (AB236415)

All lanes:

Western blot - Anti-SSX2 antibody [OTI4A11] (ab236415) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY SSX2 cDNA at 5 µg

Predicted band size: 22 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI4A11

Isotype

IgG1

Carrier free

Reacts with

Human, African green monkey

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SSX2.

Q16385

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

style

left-column

제품 세부 정보

Clone OTI4A11 (formerly 4A11).

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

Purified from cell culture supernatant.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SSX2 is a member of the synovial sarcoma X (SSX) breakpoint protein family. It performs mechanical function by acting as a transcriptional repressor. This protein also known as cancer/testis antigen 5.2 (CT5.2) has a mass of approximately 23 kDa. SSX2 expression is mostly restricted to testicular tissue in healthy adults but becomes anomalously expressed in various cancers making it a cancer/testis antigen of interest in research.

Biological function summary

SSX2 binds to other nuclear proteins suggesting it forms part of a regulatory complex affecting gene transcription. Such interactions influence cell cycle progression and other cellular processes. Given its involvement in transcriptional control SSX2 exerts significant influence over cellular proliferation and differentiation. Understanding the exact mechanisms by which SSX2 interacts with DNA and other proteins remains an area of keen scientific investigation because of its unique expression patterns.

Pathways

SSX2 impacts pathways related to transcriptional regulation and cellular growth. SSX2's involvement in the Wnt signaling pathway critical for cell proliferation illustrates its regulatory influence over cellular mechanisms. Furthermore its interaction with proteins like beta-catenin within these pathways can modulate various cellular outcomes affecting oncogenesis and tissue development.

SSX2 expression shows correlation with cancers such as melanoma and synovial sarcoma. The aberrant expression in these malignancies makes SSX2 a potential target for therapeutic interventions or a marker for diagnosis. Its link with MAGE proteins another family of cancer/testis antigens adds layers to its role in cancer biology. By better understanding SSX2's function and interactions researchers can advance therapeutic strategies to target its expression in cancerous cells.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Could act as a modulator of transcription.

See full target information SSX2

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer biology & therapy 25:2427415 PubMed39530604

2024

A positive feedback loop of SRSF9/USP22/ZEB1 promotes the progression of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Ming Hu,Jie Min,Xin Li

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SSX2 antibody [OTI4A11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Immunocytochemistry/ Immunofluorescence - Anti-SSX2 antibody [OTI4A11] (AB236415)

Western blot - Anti-SSX2 antibody [OTI4A11] (AB236415)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise