Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (AB234902)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma cell line) cell line labeling STAT1 with ab210524 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (AB234902)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling STAT1 with ab210524 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on glomerulus and some renal tubules of human kidney (PMID : 26678048) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (AB234902)

Immunohistochemical analysis of paraffin-embedded paired human endometrial cancer and non-tumor endometrium tissue labeling STAT1 with ab210524 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Much higher staining intensity of endometrial cancer (Panel A) than its paired non-tumor endometrium (Panel B) (PMID : 25267067) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (AB234902)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling STAT1 with ab210524 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining mainly on interfollicular cells of human tonsil (PMID : 25336386; PMID : 25921060) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (AB234902)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling STAT1 with ab210524 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

• IP

Supplier Data

Immunoprecipitation - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (AB234902)

STAT1 was immunoprecipitated from 0.35 mg MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab210524 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab210524 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1 : MCF7 whole cell lysate 10 μg (Input).
Lane 2 : ab210524 IP in MCF7 whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab210524 in MCF7 whole cell lysate (-).

Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 15 seconds.

The doublet observed in some lanes likely represent the α and β isoforms of STAT1 (PMID : 8647800).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

All lanes:

Immunoprecipitation - Anti-STAT1 antibody [EPRR21057-168] (<a href='/ko/products/primary-antibodies/stat1-antibody-eprr21057-168-ab210524'>ab210524</a>)

Predicted band size: 87 kDa

false

• WB

Lab

Western blot - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (AB234902)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

Western blot : Anti-STAT1 antibody [EPRR21057-168] ab210524 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 87 kDa in Wild-type HeLa cell lysates with no signal observed at this size in STAT1 knockout HeLa cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-STAT1 antibody [EPRR21057-168] (<a href='/ko/products/primary-antibodies/stat1-antibody-eprr21057-168-ab210524'>ab210524</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysates at 20 µg

Lane 2:

Western blot - Human STAT1 knockout HeLa cell line (<a href='/ko/products/cell-lines/human-stat1-knockout-hela-cell-line-ab255346'>ab255346</a>) at 20 µg

Lane 3:

A-431 cell lysates at 20 µg

Lane 4:

K562 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 87 kDa

false