AB119991

Anti-Stathmin 1 antibody [EP1572]

Name: Anti-Stathmin 1 antibody [EP1572]
Brand: Abcam Limited
SKU: AB119991
Rating: 5 (1 reviews)

RabMAb
각 태그의 뜻

(1 리뷰)

(1 출판물)

Rabbit Monoclonal Stathmin 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

C1orf215, LAP18, OP18, STMN1, Stathmin, Leukemia-associated phosphoprotein p18, Metablastin, Oncoprotein 18, Phosphoprotein p19, Prosolin, Protein Pr22, pp17, Op18, pp19

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1572] (AB119991)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1572] (AB119991)

ab119991 at 1/100 dilution staining Stathmin 1 in Paraffin-embedded Human Brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Stathmin 1 antibody [EP1572] (AB119991)

All lanes:

Western blot - Anti-Stathmin 1 antibody [EP1572] (ab119991) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Human spleen lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 17 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1572

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>Heat up to 98°C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Stathmin 1 also known as Oncoprotein 18 (Op18) is a cytoplasmic phosphoprotein weighing approximately 19 kDa. It functions primarily by regulating microtubule dynamics; it destabilizes microtubules and promotes depolymerization. Stathmin 1 achieves this by binding to tubulin heterodimers preventing their polymerization into microtubules. This protein is widely expressed in neurons immune cells and various cancer cell lines indicating its significant role in cellular processes.

Biological function summary

Stathmin 1 is important in cell cycle regulation and cell signaling. It does not operate as part of a larger protein complex but rather exerts its effects directly on microtubules. By overriding the stabilization of microtubules it ensures proper mitotic spindle function. This action is especially significant during the G2/M transition and mitosis contributing to the precise segregation of chromosomes.

Pathways

The regulation of microtubule dynamics by Stathmin 1 plays an important role in cell division and neuronal development. It is involved in the PI3K/AKT signaling pathway which ensures cell survival and proliferation. Stathmin 1 interacts with other regulatory proteins such as Tau which stabilizes microtubules offering a balance between dynamic instability and stabilization. This interaction highlights its involvement in cytoskeletal reorganization essential for both cancer progression and neuronal function.

The altered expression of Stathmin 1 is often associated with various cancers including breast and ovarian cancers. Overexpression leads to enhanced tumor growth and metastasis. It also plays a role in neurological disorders by affecting neuronal microtubules making it of particular interest in research on neurodegenerative diseases like Alzheimer's. Its interaction with proteins like Tau in the brain further connects it to these disorders as both are involved in microtubule modulation important for maintaining cell structure and function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity).

See full target information STMN1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JCI insight 6: PubMed34383713

2021

TGF-β1 protein trap AVID200 beneficially affects hematopoiesis and bone marrow fibrosis in myelofibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lilian Varricchio,Camelia Iancu-Rubin,Bhaskar Upadhyaya,Maria Zingariello,Fabrizio Martelli,Paola Verachi,Cara Clementelli,Jean-Francois Denis,Adeeb H Rahman,Gilles Tremblay,John Mascarenhas,Ruben A Mesa,Maureen O'Connor-McCourt,Anna Rita Migliaccio,Ronald Hoffman

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Stathmin 1 antibody [EP1572]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1572] (AB119991)

Western blot - Anti-Stathmin 1 antibody [EP1572] (AB119991)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise