AB236223

Anti-Stathmin 1 antibody [SP49] - BSA and Azide free

Name: Anti-Stathmin 1 antibody [SP49] - BSA and Azide free
Brand: Abcam Limited
SKU: AB236223
Rating: 1 (1 reviews)

RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(1 출판물)

Rabbit Recombinant Monoclonal Stathmin 1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

대체 명칭 보기

C1orf215, LAP18, OP18, STMN1, Stathmin, Leukemia-associated phosphoprotein p18, Metablastin, Oncoprotein 18, Phosphoprotein p19, Prosolin, Protein Pr22, pp17, Op18, pp19

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

ab101690 at 1/100 dilution staining Stathmin 1 in formalin-fixed, paraffin-embedded Human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab101690).

Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling Stathmin 1 with purified ab101690 at 1/500 (0.6 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690).

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Intracellular Flow Cytometry analysis of HepG2 (human liver hepatocellular carcinoma cell line) labeling Stathmin 1 with purified ab101690 at 1/40 dilution (7.48μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Intracellular flow cytometric analysis of rabbit anti-Stathmin 1 (SP49) antibody ab101690 (1/100) in HeLa cells (green) compared to negative control of rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690).

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labeling Stathmin 1 with purified ab101690 at 1/40 dilution (7.48μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Stathmin 1 with Purified ab101690 at 1/400 dilution (0.29 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP49

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

IHC-P, Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed bycooling at RT for 20 min.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab236223 is the carrier-free version of ab101690.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from TCS by protein A/G.

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Stathmin 1 also known as Oncoprotein 18 (Op18) is a cytoplasmic phosphoprotein weighing approximately 19 kDa. It functions primarily by regulating microtubule dynamics; it destabilizes microtubules and promotes depolymerization. Stathmin 1 achieves this by binding to tubulin heterodimers preventing their polymerization into microtubules. This protein is widely expressed in neurons immune cells and various cancer cell lines indicating its significant role in cellular processes.

Biological function summary

Stathmin 1 is important in cell cycle regulation and cell signaling. It does not operate as part of a larger protein complex but rather exerts its effects directly on microtubules. By overriding the stabilization of microtubules it ensures proper mitotic spindle function. This action is especially significant during the G2/M transition and mitosis contributing to the precise segregation of chromosomes.

Pathways

The regulation of microtubule dynamics by Stathmin 1 plays an important role in cell division and neuronal development. It is involved in the PI3K/AKT signaling pathway which ensures cell survival and proliferation. Stathmin 1 interacts with other regulatory proteins such as Tau which stabilizes microtubules offering a balance between dynamic instability and stabilization. This interaction highlights its involvement in cytoskeletal reorganization essential for both cancer progression and neuronal function.

The altered expression of Stathmin 1 is often associated with various cancers including breast and ovarian cancers. Overexpression leads to enhanced tumor growth and metastasis. It also plays a role in neurological disorders by affecting neuronal microtubules making it of particular interest in research on neurodegenerative diseases like Alzheimer's. Its interaction with proteins like Tau in the brain further connects it to these disorders as both are involved in microtubule modulation important for maintaining cell structure and function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity).

See full target information STMN1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer cell 42:444-463.e10 PubMed38428410

2024

Multi-omic profiling of follicular lymphoma reveals changes in tissue architecture and enhanced stromal remodeling in high-risk patients.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea J Radtke,Ekaterina Postovalova,Arina Varlamova,Alexander Bagaev,Maria Sorokina,Olga Kudryashova,Mark Meerson,Margarita Polyakova,Ilia Galkin,Viktor Svekolkin,Sergey Isaev,Daniil Wiebe,Anna Sharun,Alexander Sarachakov,Grigory Perelman,Yaroslav Lozinsky,Ziv Yaniv,Bradley C Lowekamp,Emily Speranza,Li Yao,Stefania Pittaluga,Arthur L Shaffer,Danny Jonigk,James D Phelan,Theresa Davies-Hill,Da Wei Huang,Pavel Ovcharov,Krystle Nomie,Ekaterina Nuzhdina,Nikita Kotlov,Ravshan Ataullakhanov,Nathan Fowler,Michael Kelly,Jagan Muppidi,Jeremy L Davis,Jonathan M Hernandez,Wyndham H Wilson,Elaine S Jaffe,Louis M Staudt,Mark Roschewski,Ronald N Germain

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Stathmin 1 antibody [SP49] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

관련 conjugated 항체와 다양한 조성의 항체 (8)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise