AB324357

Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal STEAP2 antibody. Carrier free. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357)

This data was developed using ab324346, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling STEAP2 with ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human kidney (PMID : 37966111). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357)

This data was developed using ab324346, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human STEAP2 expression vector containing a His tag. (B) HEK-293T transfected with a human STEAP1B1 expression vector containing a His tag. (C) HEK-293T transfected with a human STEAP1B2 expression vector containing a His tag. (D) HEK-293T transfected with a human STEAP1 expression vector containing a His tag. (E) HEK-293T transfected with a human STEAP3 expression vector containing a His tag. (F) HEK-293T transfected with a human STEAP4 expression vector containing a His tag. (G) HEK-293T transfected with empty vector containing a His tag. tissue labeling STEAP2 with ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a human STEAP2 expression vector containing a His tag, no staining on (B) HEK-293T transfected with a human STEAP1B1 expression vector containing a His tag, (C) HEK-293T transfected with a human STEAP1B2 expression vector containing a His tag, (D) HEK-293T transfected with a human STEAP1 expression vector containing a His tag, (E) HEK-293T transfected with a human STEAP3 expression vector containing a His tag, (F) HEK-293T transfected with a human STEAP4 expression vector containing a His tag, and (G) HEK-293T transfected with empty vector containing a His tag. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357)

This data was developed using ab324346, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling STEAP2 with ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human skeletal muscle. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357)

This data was developed using ab324346, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded : (A)LNCap (human prostate carcinoma epithelial cell) cell pellet; (B)PC3 (human prostate adenocarcinoma epithelial cell) cell pellet; (C)Hela (human cervical adenocarcinoma epithelial cell) cell pellet tissue labeling STEAP2 with ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive on A : LNCap cell pellet, no staining on (B) PC3 cell pellet and (C) Hela cell pellet. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357)

This data was developed using ab324346, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labeling STEAP2 with ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human prostate carcinoma (PMID : 37966111). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30542-566

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Punctate nuclear staining has been observed in some instances in negative control cell lines; the identity of this signal is unknown. This staining has not been observed in tissues tested.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

ab324357 is the carrier free version of ab324346.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Integral membrane protein that functions as a NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane (By similarity). Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate (By similarity). Can also reduce Cu(2+) to Cu(1+) (By similarity).

See full target information STEAP2

대체 명칭 보기

PCANAP1, STAMP1, UNQ6507/PRO23203, STEAP2, Metalloreductase STEAP2, Prostate cancer-associated protein 1, Protein up-regulated in metastatic prostate cancer, Six-transmembrane epithelial antigen of prostate 2, SixTransMembrane protein of prostate 1, PUMPCn

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise