Anti-STIM2 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-STIM2 antibody (AB241510)

STIM2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

ab241510 used for IP at 6 μg per reaction. Western blot was perfomed on the immunoprecipitate using another anti-STIM2 antibody at 1 μg/ml.

Lane 1 : ab241510 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-STIM2 antibody (ab241510)

Predicted band size: 84 kDa

false

• WB

Supplier Data

Western blot - Anti-STIM2 antibody (AB241510)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-STIM2 antibody (ab241510) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 84 kDa

true

Exposure time: 3min