Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (AB316006)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Streptococcus mutans Cell surface antigen I/II with ab316006 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human cerebrum. The section was incubated with ab316006 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (AB316006)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Streptococcus mutans Cell surface antigen I/II with ab316006 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse cerebrum.The section was incubated with ab316006 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (AB316006)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Streptococcus mutans Cell surface antigen I/II with ab316006 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat cerebrum. The section was incubated with ab316006 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (AB316006)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag® (Middle) / 293T transfected with an empty expression vector (Right) cells labelling Streptococcus mutans Cell surface antigen I/II with ab316006 at 1/5000 dilution (0.01 ug)/Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with primary antibody. Fixed with 2% PFA for 10 min followed by intracellularly staining with anti-Myc tag conjugated to Alexa Fluor® 647.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (AB316006)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®. (B) HEK-293T transfected with empty vector containing a myc-His-tag®. Tissue labeling Streptococcus mutans Cell surface antigen I/II with ab316006 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on HEK-293T transfected with a Myc-His-tagged Streptococcus mutans Cell surface antigen I/II construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with ab316006 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (AB316006)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag® cells labelling Streptococcus mutans Cell surface antigen I/II with ab316006 at 1/250 (2.024 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).

Confocal image showing positive staining in 293T transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain at 1/100 (0.38ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• I-ELISA

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Indirect ELISA - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (AB316006)

Indirect ELISA analysis of ab316006 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Streptococcus mutans Cell surface antigen I/II.

Antigen concentration : 1000 ng/ml

• IP

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Immunoprecipitation - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (AB316006)

Streptococcus mutans Cell surface antigen I/II was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®, whole cell lysate with ab316006 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316006 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®, whole cell lysate
Lane 2 : ab316006 IP in 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®, whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316006 in 293T cells transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®, whole cell lysate

All lanes:

Immunoprecipitation - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006) at 1/30 dilution

All lanes:

293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®, whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 6s

• WB

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Western blot - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (AB316006)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 4 µg

Lane 2:

293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®, whole cell lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 260 kDa,36 kDa

false

Exposure time: 3s