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AB317213

Anti-SUCLG1 antibody [HL2387]

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Rabbit Recombinant Monoclonal SUCLG1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SUCLG1.

대체 명칭 보기

Itaconyl--CoA ligase [ADP/GDP-forming] subunit alpha, Malyl--CoA ligase [ADP/GDP-forming] subunit alpha, Succinyl-CoA synthetase subunit alpha, SCS-alpha, SUCLG1

4 이미지
Immunocytochemistry/ Immunofluorescence - Anti-SUCLG1 antibody [HL2387] (AB317213)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SUCLG1 antibody [HL2387] (AB317213)

ab317213 detects SUCLG1 protein at mitochondria by immunofluorescent analysis. Sample : HeLa cells were fixed in ice-cold MeOH for 5 min. Green : SUCLG1 stained by ab317213 diluted at 1 : 500. Red : alpha Tubulin a cytoskeleton marker stained by alpha Tubulin antibody [GT114] diluted at 1 : 1000. Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody [HL2387] (AB317213)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody [HL2387] (AB317213)

ab317213 detects SUCLG1 protein at mitochondria by immunohistochemical analysis. Sample : Paraffin-embedded human kidney. SUCLG1 stained by ab317213 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody [HL2387] (AB317213)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody [HL2387] (AB317213)

ab317213 detects SUCLG1 protein at mitochondria by immunohistochemical analysis. Sample : Paraffin-embedded rat tissue. SUCLG1 stained by ab317213 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody [HL2387] (AB317213)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody [HL2387] (AB317213)

ab317213 detects SUCLG1 protein at mitochondria by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissue. SUCLG1 stained by ab317213 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2387

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SUCLG1.

P53597

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보완 제품

Primary Antibodies

AB14734

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Lab Essentials|KO Validated

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

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Primary Antibodies

AB244263

Anti-TXNDC/TMX antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

  • 0
  • 0
Primary Antibodies

AB321893

Anti-NDUFV1 antibody [EPR29179-78]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (AB321893)

  • 0
  • 0
Primary Antibodies

AB186735

Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)

  • 5
  • 16
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SUCLG1 also known as succinate-CoA ligase (GDP-forming) subunit alpha participates in cellular energy metabolism. It catalyzes the conversion of succinyl-CoA to succinate in the mitochondria linking the citric acid cycle and oxidative phosphorylation. SUCLG1 has a molecular mass of approximately 32 kDa. This protein expresses highly in metabolically active tissues such as the liver kidney and brain where energy demand is significant.
Biological function summary

Succinate-CoA ligase facilitates the synthesis of nucleoside triphosphates within the mitochondria by participating in the GDP-specific form of the enzyme complex. SUCLG1 forms a heterodimer with SUCLG2 (the beta subunit) creating succinate-CoA ligase's functional enzyme complex. Through its action SUCLG1 directly impacts the generation of GTP which plays a critical role in modulating metabolic reactions within cells.

Pathways

Succinate-CoA ligase integrates into the citric acid cycle and plays a significant role in cellular respiration. By converting succinyl-CoA to succinate it helps sustain the cycle's continued operation. The protein also links with nucleoside diphosphate kinase which governs the maintenance of cellular nucleotide balance. These interactions highlight the essential nature of SUCLG1 in maintaining energy flow within cells.

Mutations in SUCLG1 associate with mitochondrial DNA depletion syndromes which severely affect muscle and brain tissue. Dysfunction within succinate-CoA ligase disrupts the energy production process leading to cellular energy deficits. Additionally this protein’s interaction with SUCLG2 implies potential implications in similar disorders related to mitochondrial function showing the importance of their combined activity in maintaining cellular health.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA (PubMed : 34492704, PubMed : 40108300). The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits (By similarity). Also able to act as an itaconyl- and malyl-CoA synthetase (PubMed : 40108300).
See full target information SUCLG1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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