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AB243069

Anti-Sulfotyrosine antibody [12B11]

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Mouse Monoclonal Sulfotyrosine antibody. Suitable for WB, IHC-P and reacts with Chemical samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to Sulfotyrosine.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfotyrosine antibody [12B11] (AB243069)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfotyrosine antibody [12B11] (AB243069)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Sulfotyrosine using ab243069 at 1/25 dilution for 1 hour at room temperature in immunohistochemical analysis (C). The nuclear counterstain is DAPI (blue, A). Actin is detected with Actin-binding Phalloidin-AlexaFluor® 633 (red, B). (D) is a composite image.

Secondary Antibody : Goat Anti-Mouse IgG : AlexaFluor® 488.

Western blot - Anti-Sulfotyrosine antibody [12B11] (AB243069)
  • WB

Unknown

Western blot - Anti-Sulfotyrosine antibody [12B11] (AB243069)

Primary incubation for 2 hours at room temperature.

Secondary incubation for 1 hour at room temperature.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-Sulfotyrosine antibody [12B11] (ab243069) at 1/1000 dilution

Lane 1:

Sulfotyrosine-BSA at 2 µg

Lane 2:

Tyrosine-BSA at 2 µg

Lane 3:

Phosphotyrosine-BSA at 2 µg

Lane 4:

BSA at 2 µg

Secondary

Lanes 1 - 3:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

Lane 4:

Goat Anti-Mouse IgG at 1/2000 dilution

true

Exposure time: 5min

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

12B11

Isotype

IgG1

Carrier free

No

Applications

WB, IHC-P

applications

Specificity

<p>Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.</p>

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대체품

Primary Antibodies

AB136481

Anti-Sulfotyrosine antibody [Sulfo-1C-A2]

Western blot - Anti-Sulfotyrosine antibody [Sulfo-1C-A2] (AB136481)

  • 0
  • 0

보완 제품

Primary Antibodies

AB136481

Anti-Sulfotyrosine antibody [Sulfo-1C-A2]

Western blot - Anti-Sulfotyrosine antibody [Sulfo-1C-A2] (AB136481)

  • 0
  • 0
Secondary Antibodies

AB205719

Goat Anti-Mouse IgG H&L (HRP)

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB205719)

  • 5
  • 4
Primary Antibodies

AB20181

Anti-HLA-DR antibody [TAL 1B5]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [TAL 1B5] (AB20181)

  • 5
  • 6
Primary Antibodies

AB66217

Anti-ABCA1 antibody [HJ1]

KO Validated|BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] (AB66217)

  • 3
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

The clone number has been updated from 7C5 to 12B11, both clone numbers name the same antibody clone.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purified from TCS.
보관 버퍼
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Sulfotyrosine is a post-translational modification of the amino acid tyrosine where a sulfate group gets added to the phenolic hydroxyl moiety. This process is known as tyrosine sulfation and plays a critical role in protein-protein interactions. Tyrosine sulfation occurs primarily in the Golgi apparatus and is mediated by tyrosylprotein sulfotransferases. It doesn't refer to a single protein but rather the chemical modification found on various proteins. The distinct molecular mass for the modification itself is about 80 Da taking into account the addition of the sulfate group. This modification mostly appears in secretory and membrane proteins across a wide range of tissues and organisms.
Biological function summary

The sulfotyrosine modification significantly enhances the binding properties of proteins involved in diverse biological processes. It affects proteins such as adhesion molecules G protein-coupled receptors and coagulation factors often being essential for their proper function. Many proteins containing sulfotyrosine participate in forming multi-protein complexes that mediate cell signaling and communication.

Pathways

Sulfotyrosine modifications integrate notably into signal transduction and immune response pathways. Proteins like P-selectin glycoprotein ligand-1 (PSGL-1) and chemokine receptors such as CCR5 require sulfotyrosine for optimal receptor-ligand interactions which impacts leukocyte trafficking and chemotaxis. These modifications often serve as docking sites for other proteins coordinating complex pathways important for cellular responses.

Sulfotyrosine modifications have implications in inflammatory diseases and cancer. The modification plays a role in mediating the interactions involving proteins like PSGL-1 in immune response pathways which are critical in inflammatory diseases such as rheumatoid arthritis. Additionally abnormal tyrosine sulfation on chemokine receptors like CCR5 has associations with the metastatic properties of cancer influencing both tumor development and immune evasion mechanisms. Their correlation emphasizes the importance of understanding sulfotyrosine's functional roles in disease pathogenesis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information Sulfotyrosine
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in pharmacology 16:1617185 PubMed40926987

2025

Renoprotective effects of GHRH agonist MR409 is associated with reduced oxidative stress and ferroptosis in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyang Liu,Rong Fu,Qi Tang,Yaoxia Zhang,Ruiping Cai,Limin Liu,Hui Jia,Junjia Gao,Ming-Sheng Zhou
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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