AB190757

Anti-Sumo 2 + Sumo 3 antibody [SM23/496]

Name: Anti-Sumo 2 + Sumo 3 antibody [SM23/496]
Brand: Abcam Limited
SKU: AB190757
Rating: 3 (1 reviews)

(1 리뷰)

(2 제품이 사용된 논문 )

Mouse Monoclonal Sumo 2 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SUMO2.

대체 명칭 보기

SMT3B, SMT3H2, SUMO2, Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B, SMT3A, SMT3H1, SUMO3, Small ubiquitin-related modifier 3, SUMO-3, SMT3 homolog 1, SUMO-2, Ubiquitin-like protein SMT3A, Smt3A

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [SM23/496] (AB190757)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [SM23/496] (AB190757)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Sumo 2+3 using ab190757 at 1 μg/ml.

Supplier Data

Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [SM23/496] (AB190757)

Immunofluorescence staining of paraformaldehyde-fixed MCF-7 cells labeling Sumo 2 + Sumo 3 with ab190757 followed by goat anti-Mouse IgG-CF488 (Green). Membrane are labeled with Phalloidin (Red).

Supplier Data

Western blot - Anti-Sumo 2 + Sumo 3 antibody [SM23/496] (AB190757)

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 antibody [SM23/496] (ab190757) at 1 µg/mL

All lanes:

HeLa cell lysate

Predicted band size: 12 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

SM23/496

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

ICC, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SUMO2.

P61956

Specificity

This antibody reacts with both SUMO2 and SUMO3.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 관련 사항

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Sumo 2 and Sumo 3 are small ubiquitin-like modifier proteins involved in post-translational modification often referred to as SUMOylation. Alternately known as SUMO-2 and SUMO-3 these proteins weigh approximately 11 kDa each and are widely expressed across various tissues including the heart and brain. During stress conditions cells significantly increase the conjugation of these proteins to other cellular proteins thereby altering their functions subcellular localization or interactions.

Biological function summary

Sumo 2 and Sumo 3 play essential roles in numerous cellular processes by modifying several target proteins in the nucleus and cytoplasm. They are part of a complex regulatory system that modulates processes like nuclear transport chromosome segregation and DNA repair. Sumo 2/3 often work in tandem with other members of the SUMO family including SUMO-1 to ensure proper cellular homeostasis.

Pathways

Sumo 2 and Sumo 3 are intimately involved in fundamental cellular signaling pathways such as the p53 pathway and NF-κB pathway. These proteins modulate the activity of key proteins within these pathways including the tumor suppressor p53 and the transcription factor NF-κB which regulate cell survival proliferation and apoptosis. Sumo 2/3-related modifications can alter the stability and function of these proteins thereby influencing cellular response to stimuli.

Alterations in Sumo 2 and Sumo 3 functionality have been associated with neurodegenerative diseases and various cancers. In neurodegenerative disorders like Huntington's disease abnormal SUMOylation of specific proteins affects neuronal survival and function. Similarly in cancer dysregulation of SUMOylation pathways can affect the expression and activity of the oncogenic protein c-Myc contributing to tumor progression. These associations underline the significant role of Sumo 2/3 in pathophysiology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed : 26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed : 18408734, PubMed : 18538659, PubMed : 21965678, PubMed : 9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed : 24105744).

See full target information SUMO2

Additional targets

SUMO3

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nucleic acids research 52:11738-11752 PubMed39268578

2024

Mechanism of BCDX2-mediated RAD51 nucleation on short ssDNA stretches and fork DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Akita,Paul Girvan,Mario Spirek,Jiri Novacek,David Rueda,Zbynek Prokop,Lumir Krejci

Oncology reports 44:1355-1364 PubMed32945483

2020

SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Tao Liu,Shutao Zheng,Qing Liu,Tongxue Shen,Xiujuan Han,Qiqi Zhang,Lifei Yang,Xiaomei Lu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sumo 2 + Sumo 3 antibody [SM23/496]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [SM23/496] (AB190757)

Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [SM23/496] (AB190757)

Western blot - Anti-Sumo 2 + Sumo 3 antibody [SM23/496] (AB190757)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

제품이 사용된 논문 (2)

Product promise