Anti-SUR1 antibody [EPR25761-44] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SUR1 antibody [EPR25761-44] - BSA and Azide free (AB302612)

This data was developed using ab302611, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-N-BE(2) (human neuroblastoma neuroblast) cells labelling SUR1 with ab302611 at 1/100 (5.37 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in SK-N-BE(2) cell lineNegative control : HEK-293 (PMID : 23255597) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SUR1 antibody [EPR25761-44] - BSA and Azide free (AB302612)

This data was developed using ab302611, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293 (human embryonic kidney epithelial cell, Left) / SK-N-BE(2) (human neuroblastoma neuroblast, Right) cells labelling SUR1 with ab302611 at 1/5000 dilution (0.01ug) (Red) compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : HEK-293 (PMID : 23255597).

• WB

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Western blot - Anti-SUR1 antibody [EPR25761-44] - BSA and Azide free (AB302612)

This data was developed using ab302611, the same antibody clone in a different buffer.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Kidney (PMID : 17535866 ), HEK-293(PMID : 23255597, 32332165 )

The expression molecular weight observed in lane 1 is consistent with what has been described in the literature (PMID : 21674179, 17657312)

Samples are non-boiled as boiling may cause protein aggregates.

Lane 1 of this blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

Exposure time :

Lane 1 : 3 minutes

Lane 2, 3, 6, 7 : 26 seconds

Lane 4, 5 : 70 seconds

All lanes:

Western blot - Anti-SUR1 antibody [EPR25761-44] (<a href='/ko/products/primary-antibodies/sur1-antibody-epr25761-44-ab302611'>ab302611</a>) at 1/1000 dilution

Lane 1:

Human pancreas tissue lysate 20 µg at 20 µg

Lane 2:

Human hypothalamus tissue lysate 20 µg at 20 µg

Lane 3:

Human kidney tissue lysate 20 µg at 20 µg

Lane 4:

SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate 20 µg at 20 µg

Lane 5:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate 20 µg at 20 µg

Lane 6:

Rat hypothalamus tissue lysate 20 µg at 20 µg

Lane 7:

Rat kidney tissue lysate 20 µg at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa,180 kDa

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