AB72569

SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

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(13 제품이 사용된 논문 )

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] conjugated to SureLight® APC - suitable for FRET, intracellular flow cytometry samples. Clone [M2] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72569)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72569)

Flow Cytometry analysis of transfected HEK293 cells. Fixed and permeabilized cells stained with 1 μg/ml of ab72569

Shaded histogram – HEK293 cells, expressing DYKDDDDK-tagged protein
Open histogram – mock transfected control HEK293 cellsNo exactly the same info

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Conjugation

SureLight® APC

Excitation/Emission

Ex: 652nm, Em: 657nm

Carrier free

Applications

Flow Cyt (Intra), FRET

applications

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FRET" : {"fullname" : "Fluorescence Resonance Energy Transfer", "shortname":"FRET"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "FRET-species-checked": "guaranteed", "FRET-species-dilution-info": "", "FRET-species-notes": "<p></p>" }, "Transfected cell lysate": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "FRET-species-checked": "notRecommended", "FRET-species-dilution-info": "", "FRET-species-notes": "" } } }

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제품 세부 정보

What is this antibody validated in?
SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt). Tagged protein detection
DDDDK tag (Binds to FLAG® tag sequence) antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Related products
Antibody clone M2 is also available pre-conjugated to a variety of labels for your convenience – Anti-DDDDK tag (Binds to FLAG® tag sequence) SureLight® APC [M2] (ab72569).

Other related products
We have a range of other formats of antibody clone [M2] also available for your convenience: Alkaline Phosphatase - ab81655, DyLight® 650 - ab117492, DyLight® 550 - ab117495, Carrier free - ab117505, PerCP - ab117514, Europium - ab187914

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Size-exclusion chromatography

Purification 관련 사항

Purified using size exclusion chromatography as well as some proprietary methods for purifying the final materials.

보관 버퍼

pH: 7.4 Preservative: 0.013% Sodium azide Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Store in the dark

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information DDDDK tag (Binds to FLAG® tag sequence)

대체 명칭 보기

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk, flag, flag tag

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제품이 사용된 논문 (13)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:8403 PubMed40998787

2025

Structural basis for ligand promiscuity and high signaling activity of Kaposi's Sarcoma-associated Herpesvirus-encoded GPCR.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Bae Park,Bibekananda Sahoo,Amita Rani Sahoo,Dokyun Kim,Hogyu David Seo,James Bowman,Mi-Jeong Kwak,Sophia Suh,Matthias Buck,Xinghong Dai,Jae U Jung

Nature chemical biology 21:1719-1730 PubMed40846994

2025

Conversion of natural cytokine receptors into orthogonal synthetic biosensors.

Applications

Unspecified application

Species

Unspecified reactive species

Hailey I Edelstein,Amparo Cosio,Max L Ezekiel,William K Corcoran,Aaron H Morris,Joshua N Leonard

Cell 187:5554-5571.e19 PubMed39197450

2024

Mosaic sarbecovirus nanoparticles elicit cross-reactive responses in pre-vaccinated animals.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander A Cohen,Jennifer R Keeffe,Ariën Schiepers,Sandra E Dross,Allison J Greaney,Annie V Rorick,Han Gao,Priyanthi N P Gnanapragasam,Chengcheng Fan,Anthony P West,Arlene I Ramsingh,Jesse H Erasmus,Janice D Pata,Hiromi Muramatsu,Norbert Pardi,Paulo J C Lin,Scott Baxter,Rita Cruz,Martina Quintanar-Audelo,Ellis Robb,Cristina Serrano-Amatriain,Leonardo Magneschi,Ian G Fotheringham,Deborah H Fuller,Gabriel D Victora,Pamela J Bjorkman

Nature biomedical engineering 8:397-414 PubMed38012307

2023

Genetically encoding multiple functionalities into extracellular vesicles for the targeted delivery of biologics to T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Devin M Stranford,Lacy M Simons,Katherine E Berman,Luyi Cheng,Beth N DiBiase,Michelle E Hung,Julius B Lucks,Judd F Hultquist,Joshua N Leonard

eLife 11: PubMed36468682

2022

Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Jazlyn P Borges,Ragnhild S R Sætra,Allen Volchuk,Marit Bugge,Pascal Devant,Bjørnar Sporsheim,Bridget R Kilburn,Charles L Evavold,Jonathan C Kagan,Neil M Goldenberg,Trude Helen Flo,Benjamin Ethan Steinberg

Synthetic biology (Oxford, England) 5:ysaa017 PubMed33392392

2020

Elucidation and refinement of synthetic receptor mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Hailey I Edelstein,Patrick S Donahue,Joseph J Muldoon,Anthony K Kang,Taylor B Dolberg,Lauren M Battaglia,Everett R Allchin,Mihe Hong,Joshua N Leonard

mAbs 12:1684749 PubMed31775561

2019

Development of a high yielding expression platform for the introduction of non-natural amino acids in protein sequences.

Applications

Unspecified application

Species

Unspecified reactive species

Gargi Roy,Jason Reier,Andrew Garcia,Tom Martin,Megan Rice,Jihong Wang,Meagan Prophet,Ronald Christie,William Dall'Acqua,Sanjeev Ahuja,Michael A Bowen,Marcello Marelli

The Journal of biological chemistry 293:17505-17522 PubMed30242129

2018

Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhiro Hayashi,Yoko Nemoto-Sasaki,Naoki Matsumoto,Kotaro Hama,Takashi Tanikawa,Saori Oka,Tadaaki Saeki,Tatsuya Kumasaka,Takanori Koizumi,Seisuke Arai,Ikuo Wada,Kazuaki Yokoyama,Takayuki Sugiura,Atsushi Yamashita

mAbs 10:416-430 PubMed29400603

2018

A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Gargi Roy,Tom Martin,Arnita Barnes,Jihong Wang,Rod Brian Jimenez,Megan Rice,Lina Li,Hui Feng,Shu Zhang,Raghothama Chaerkady,Herren Wu,Marcello Marelli,Diane Hatton,Jie Zhu,Michael A Bowen

Nature chemical biology 13:202-209 PubMed27941759

2016

Rewiring human cellular input-output using modular extracellular sensors.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly A Schwarz,Nichole M Daringer,Taylor B Dolberg,Joshua N Leonard

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72569)

관련 conjugated 항체와 다양한 조성의 항체 (8)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (13)

Product promise