Anti-Survivin antibody
4
(10 리뷰들)
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(146 제품이 사용된 논문 )
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (AB469)
Paraffin-embedded human rectal cancer tissue stained for Survivin using ab469 at 0.5 μg/ml in immunohistochemical analysis, using DAB with hematoxylin counterstain.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (AB469)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Survivin (green) using ab469 at 1/10 dilution in ICC/IF. An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
- ICC/IF
AbReview2567****
Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (AB469)
HeLa cells (ab150035) in prometaphase, metaphase and anaphase stained with anti-Survivin (green), anti-tubulin (red) and DAPI (blue). These images were kindly supplied as part of the review submitted by William Moore, University of Dundee, UK.
This image is courtesy of William Moore
- ICC/IF
AbReview4371****
Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (AB469)
ab469 at a 1/400 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then was detected using a Texas Red conjugated Goat anti-rabbit antibody.
This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 30 March 2006.
- IHC-P
AbReview5207****
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (AB469)
ab469 staining Survivin from Human Ovarian carcionoma tumour tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed (Citrate buffer pH=6, microwave oven) and the tissue was then formaldehyde fixed and blocked (Hydrogen peroxide 0.03%). An HRP conjugated goat anti-rabbit was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Dr Ben Davidson
- IHC-P
AbReview6882****
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (AB469)
Paraformaldehyde-fixed, paraffin-embedded human colon carcinoma tissue stained for Survivin using ab469 at 1/500 dilution in immunohistochemical analysis.
This image is courtesy of an Abreview submitted by Mr. Rudolf Jung.
- WB
Supplier Data
Western blot - Anti-Survivin antibody (AB469)
All lanes:
Western blot - Anti-Survivin antibody (ab469) at 1 µg/mL
All lanes:
HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Predicted band size: 16 kDa,18 kDa,31 kDa,9 kDa
true
Exposure time: 1min
- WB
Unknown
Western blot - Anti-Survivin antibody (AB469)
All lanes:
Western blot - Anti-Survivin antibody (ab469) at 1 µg/mL
Lane 1:
HeLa Nuclear at 20 µg
Lane 2:
HeLa whole cell lysate at 20 µg
Lane 3:
A431 cell lysate at 20 µg
Lane 4:
Jurkat cell lysate at 20 µg
Lane 5:
HEK293 cell lysate at 20 µg
Secondary
All lanes:
Alexa Fluor anti-rabbit at 1/5000 dilution
Predicted band size: 16 kDa
Observed band size: 18 kDa,37 kDa,50 kDa
false
- WB
CiteAb
Western blot - Anti-Survivin antibody (AB469)
Western Blotting using Anti-Survivin antibody, ab469. Publication image from Vaculová, A. et al., 2010, Mol Cancer, 20416058. Legend direct from paper.
SCLC cell lines significantly differ in expression of proteins involved in TRAIL signaling. The level of (A) pro-caspase-8, -2, -10, (B) FADD, cFLIPL, cFLIPS, and (C) survivin, Mcl-1, and Bcl-2 proteins in H69, H82, U1285, U1690, and U1906 cells. An equal loading was controlled using anti-β-actin antibody. Jurkat cells were used as a positive control for expression of caspase-10. Results are representative of three independent experiments.
false
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Survivin aids cell division and regulates apoptosis. It plays a major role as a chromosomal passenger protein functioning in concert with other members of the chromosomal passenger complex including Aurora B kinase INCENP and Borealin. During mitosis Survivin helps ensure proper chromosome alignment segregation and cytokinesis. Its ability to inhibit apoptosis allows it to enhance cell survival contributing to tumor progression when deregulated.
Pathways
Survivin is part of the cell cycle regulation and apoptosis pathways. It plays an integral role in the mitotic spindle checkpoint working closely with Aurora B kinase to regulate mitosis. Additionally the protein interfaces with the extrinsic apoptosis pathway interacting with members like XIAP to prevent caspase activation and cell death. These interactions reveal Survivin's dual function in promoting mitosis and regulating apoptosis which is essential for maintaining cellular homeostasis.
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제품이 사용된 논문 (146)
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