AB117702

Anti-Synaptopodin antibody

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(5 제품이 사용된 논문 )

Rabbit Polyclonal Synaptopodin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SYNPO conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

KIAA1029, SYNPO, Synaptopodin

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody (AB117702)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody (AB117702)

Human Perivascular Processes, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117702 at 5μg/ml.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody (AB117702)

Human Perineural Processes, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117702 at 5μg/ml.

CiteAb

Immunohistochemistry - Anti-Synaptopodin antibody (AB117702)

Immunohistochemistry using Anti-Synaptopodin antibody, ab117702. Publication image from Subramanian, A. et al., 2019, Nat Commun, 31784515. Legend direct from paper.

IF validation of markers derived from the single-cell data in mature kidney organoids. a IF staining of an entire kidney organoid with segment specific markers as shown. b Schematic of kidney nephron with major cell types and canonical markers annotated. c Immunofluorescence staining of D29 kidney organoids for podocyte (WT1), proximal tubule (LTL), and distal tubule (CDH1 and GATA3) across two protocols (JB, ML) and four cell lines (AS, N1, N2, ThF). IF staining for validation of markers identified in the single-cell data : d NPHS1 colocalized with the podocyte-specific marker SYNPO and e LRP2 colocalized with the proximal tubular marker LTL (bottom). f IF staining validation for MEIS1-positive mesenchymal cells in D29 organoids. LAMA1 indicates basement membranes. MEIS1 staining of mesenchymal cells appropriately surrounds LAMA1-defined tubular nephron structures.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SYNPO conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8N3V7

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Synaptopodin also known as SYNPO is an actin-associated protein with a molecular mass of approximately 100 kDa. It plays a mechanical role by associating and bundling actin filaments important for cytoskeletal dynamics. Synaptopodin gets expressed mainly in podocytes of the kidney as well as in certain neurons particularly found in dendritic spines and telencephalic dendrites. This protein is sensitive to calcium signaling which influences its interaction with the actin cytoskeleton.

Biological function summary

The actin-modulating effects of Synaptopodin contribute to the regulation of cell shape and motility. Synaptopodin is essential for the formation of the dendritic spine apparatus working as a part of a protein complex that stabilizes dendritic spines which are key to synaptic plasticity and signal transduction. The protein can also influence the podocyte function in the kidney maintaining the structure of foot processes that are fundamental for the kidney filtration barrier.

Pathways

Synaptopodin plays an important role in the maintenance of synaptic plasticity and the actin cytoskeleton pathway which affects both neuronal plasticity and kidney function. Within these pathways Synaptopodin interacts with proteins like alpha-actinin and myosin-Va forming linkages that stabilize actin filaments and regulate cytoskeletal organization. The protein contributes to the protection of neurons and kidney glomeruli through signaling pathways that affect actin dynamic balance.

Synaptopodin has significant implications in nephrotic syndrome and Alzheimer's disease. In nephrotic syndrome alterations in Synaptopodin expression or function can lead to podocyte injury contributing to kidney dysfunction. In Alzheimer's disease Synaptopodin modulation of spine morphology may impact synaptic connectivity and cognitive function. The protein interacts with nephrin in kidney pathology and potentially affects tau protein signaling in Alzheimer's illustrating its vital role in both neurological and renal health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity (By similarity).

See full target information SYNPO

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 23: PubMed35886848

2022

Differences in Immunohistochemical and Ultrastructural Features between Podocytes and Parietal Epithelial Cells (PECs) Are Observed in Developing, Healthy Postnatal, and Pathologically Changed Human Kidneys.

Applications

Unspecified application

Species

Unspecified reactive species

Marin Ogorevc,Ivona Kosovic,Natalija Filipovic,Ivana Bocina,Marija Juric,Benjamin Benzon,Snjezana Mardesic,Katarina Vukojevic,Marijan Saraga,Boris Kablar,Mirna Saraga-Babic

Scientific reports 10:8756 PubMed32471989

2020

Spatio-temporal patterning of different connexins in developing and postnatal human kidneys and in nephrotic syndrome of the Finnish type (CNF).

Applications

Unspecified application

Species

Unspecified reactive species

Ivona Kosovic,Natalija Filipovic,Benjamin Benzon,Katarina Vukojevic,Marijan Saraga,Merica Glavina Durdov,Ivana Bocina,Mirna Saraga-Babic

Nature communications 10:5462 PubMed31784515

2019

Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Ayshwarya Subramanian,Eriene-Heidi Sidhom,Maheswarareddy Emani,Katherine Vernon,Nareh Sahakian,Yiming Zhou,Maria Kost-Alimova,Michal Slyper,Julia Waldman,Danielle Dionne,Lan T Nguyen,Astrid Weins,Jamie L Marshall,Orit Rosenblatt-Rosen,Aviv Regev,Anna Greka

PeerJ 7:e8016 PubMed31799068

2019

Long noncoding RNA MEG3 suppresses podocyte injury in diabetic nephropathy by inactivating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiajing Che,Xin Deng,Kewei Xie,Qin Wang,Jiayi Yan,Xinghua Shao,Zhaohui Ni,Liang Ying

Cellular physiology and biochemistry : internation 48:1-15 PubMed29996125

2018

Activation of the Nrf2-ARE Pathway Ameliorates Hyperglycemia-Mediated Mitochondrial Dysfunction in Podocytes Partly Through Sirt1.

Applications

Unspecified application

Species

Unspecified reactive species

Qunzi Zhang,Qiongxia Deng,Jun Zhang,JianTing Ke,Ye Zhu,Ruo Wei Wen,Zengchun Ye,Hui Peng,Zhong Zhen Su,Cheng Wang,Tanqi Lou

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Synaptopodin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody (AB117702)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody (AB117702)

Immunohistochemistry - Anti-Synaptopodin antibody (AB117702)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise