AB108967

Anti-Talin 2 antibody [EPR2536(2)]

RabMAb
Recombinant
각 태그의 뜻

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(4 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Talin 2 antibody. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human samples. Cited in 4 publications.

대체 명칭 보기

KIAA0320, TLN2, Talin-2

4 이미지

Unknown

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (AB108967)

All lanes:

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution

Lane 1:

COS-1 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 123 kDa,159 kDa,271 kDa,52 kDa,54 kDa,69 kDa,70 kDa,93 kDa

Observed band size: 159 kDa,270 kDa,70 kDa

false

Lab

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (AB108967)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 271 kDa

Observed band size: 270 kDa

false

Lab

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (AB108967)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 271 kDa

Observed band size: 270 kDa

false

Lab

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (AB108967)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution

All lanes:

COS-1 cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 271 kDa

Observed band size: 270 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2536(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, African green monkey

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Talin 2 also known as TLN2 is a large cytoskeletal protein approximately 270 kDa in mass. It acts mechanically as an important player in linking integrins to the actin cytoskeleton supporting the structural integrity of cells. Talin 2 is expressed in various tissues including muscle and neuronal systems with expression patterns similar to Talin 1 but has distinct roles in cellular adhesion and signal transduction.

Biological function summary

Talin 2 plays a significant role in cell adhesion and migration processes. It is part of a complex that includes integrins and other cytoskeletal proteins facilitating the transmission of mechanical signals across the cell membrane. This protein aids in focal adhesion assembly ensuring proper cellular responses to external stimuli. Talin 2 contributes to stabilizing intracellular interactions which are essential for maintaining tissue structure and cell motility.

Pathways

Talin 2 is deeply embedded in integrin-mediated signaling and the cytoskeletal organization pathway. It modulates interactions between integrins and F-actin orchestrating actin dynamics and contributing to cellular adherence junctions. Talin 2 functions closely with proteins like vinculin which supports actin filament linkage and reinforces mechanical stability under tension.

Talin 2 has been linked to muscular dystrophies and certain neuropathies. Mutations or dysregulation in Talin 2 can impair muscle function as its interaction with dystrophin is critical for muscle integrity. Additionally disruptions in Talin 2 mediated signaling could contribute to neurodegenerative disorders where its interactions with integrins can affect neuronal connectivity and survival. Understanding Talin 2's role in these conditions may provide insights for therapeutic interventions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity (By similarity).

See full target information TLN2

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2501046 PubMed40279639

2025

Mechanical Load-Induced Upregulation of Talin2 through Non-Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhen Huang,Heng Sun,Haojie Chen,Xiangpeng Wang,Junduo Zhao,Yang Jiao,Hongyi Zhou,Haoyu Cai,Jiafeng Dai,Xuan Huang,Weiyun Chen,Jianxiong Shen

Journal of oncology 2020:8681361 PubMed32256587

2020

Long Noncoding RNA Lnc-TLN2-4:1 Suppresses Gastric Cancer Metastasis and Is Associated with Patient Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Yuyun Wu,Ningbo Hao,Suming Wang,Xin Yang,Yufeng Xiao,Huan Yang,Shiming Yang,Bosheng Li

Viruses 8: PubMed27775586

2016

Hepatitis B Virus Protein X Induces Degradation of Talin-1.

Applications

Species

Human

Maarten A A van de Klundert,Maartje van den Biggelaar,Neeltje A Kootstra,Hans L Zaaijer

Oncogene 32:127-34 PubMed22310291

2012

DNA methylation silences miR-132 in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

A Formosa,A M Lena,E K Markert,S Cortelli,R Miano,A Mauriello,N Croce,J Vandesompele,P Mestdagh,E Finazzi-Agrò,A J Levine,G Melino,S Bernardini,E Candi

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Talin 2 antibody [EPR2536(2)]

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (AB108967)

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (AB108967)

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (AB108967)

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (AB108967)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise