Anti-TARC/CCL17 antibody [EPR28961-9]

• WB

Supplier Data

Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (AB323800)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of TARC/CCL17 is upregulated in response to LPS and IL-4 treatment (PMID : 18004070).

The identity of the bands higher than 37 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (ab323800) at 1/1000 dilution

Lane 1:

Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 30 µg

Lane 2:

RAW 264.7 treated with 100ng/ml LPS for 24 hours, 10ng/ml IL-4 for 20 hours and 300ng/ml BFA for 4 hours whole cell lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 11 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (AB323800)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of TARC/CCL17 is upregulated in response to M-CSF, GM-CSF and BFA treatment (PMID : 27525438).

In Western blot, Anti-Integrin alpha V antibody [EPR16800] staining at 1/5000 dilution.

All lanes:

Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (ab323800) at 1/1000 dilution

Lane 1:

Untreated Mouse bone marrow lysate at 20 µg

Lane 2:

Mouse bone marrow treated with 100ng/mL M-CSF for 7 days, 20ng/ml GM-CSF and 300ng/ml BFA for 16 hours lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 11 kDa,125 kDa

false

Exposure time: 180s