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AB288599

Anti-TARC/CCL17 antibody [YCA-R21766-150 H4L3] - BSA and Azide free (Capture)

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Anti-TARC/CCL17 antibody [YCA-R21766-150 H4L3] - BSA and Azide free (Capture) (ab288599) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting TARC/CCL17 in ELISA. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
1 이미지
Sandwich ELISA - Anti-TARC/CCL17 antibody [YCA-R21766-150 H4L3] - BSA and Azide free (Capture) (AB288599)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-TARC/CCL17 antibody [YCA-R21766-150 H4L3] - BSA and Azide free (Capture) (AB288599)

Example of Human TARC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

YCA-R21766-150 H4L3

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB183366

Human TARC ELISA Kit (CCL17)

SimpleStep

Sandwich ELISA - Human TARC ELISA Kit (CCL17) (AB183366)

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  • 0
Sample Prep & Detection Kits

AB201801

DyLight® 594 Conjugation Kit (Fast) - Lightning-Link®

Fluorescence Microscopy - DyLight® 594 Conjugation Kit (Fast) - Lightning-Link® (AB201801)

  • 0
  • 0
Sample Prep & Detection Kits

AB269902

Gold-Maleimide Conjugation Kit (20nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (20nm, 20 OD) (AB269902)

  • 0
  • 0
Primary Antibodies

AB172730

Rabbit IgG, monoclonal [EPR25A] - Isotype Control

KD Validated|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

  • 0
  • 0
Assay Kits

AB289696

Human TARC ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human TARC ELISA Kit (AB289696)

  • 0
  • 0
Primary Antibodies

AB108443

Anti-GLP-1 antibody [EPR4042-1]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] (AB108443)

  • 1
  • 1
Primary Antibodies

AB133353

Anti-IL-13 antibody [EPR3267]

20ul selling size|RabMAb|Recombinant

ELISA - Anti-IL-13 antibody [EPR3267] (AB133353)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab288599 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human TARC/CCL17. The recommended pair for sandwich ELISA is:
Capture: ab288599, Human TARC/CCL17Capture Antibody (unconjugated)
Detector: ab288617, Human TARC/CCL17Detector Antibody (unconjugated)
The reference range value is 312.5 - 20000 pg/mL

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TARC also known as CCL17 is a chemokine with a molecular mass of approximately 8 kDa. It is mainly expressed in the thymus but also found in the lung liver and lymph nodes. CCL17 plays an important role in immune response by signaling through the CCR4 receptor. It functions as a ligand that guides the movement of T-helper 2 (Th2) cells regulatory T cells (Tregs) and other immune cells to sites of inflammation.
Biological function summary

CCL17 acts as a chemotactic agent attracting specific immune cells especially T lymphocytes to inflammatory regions. This protein does not operate as part of a larger protein complex but engages directly by binding to receptors on the target immune cells. Localized expression of CCL17 increases in conditions where enhanced Th2 cell response is needed such as allergic or autoimmune responses.

Pathways

CCL17 plays a significant role in the cell signaling pathway called the chemokine signaling pathway. Through this pathway CCL17 interacts with the CCR4 receptor which helps mediate immune cell migration and adhesion. This interaction is critical for establishing the adaptive immune response. CCL17 and CCR4 support the trafficking of Th2 cells during immune reactions and pathogen defense.

CCL17 associates with asthma and atopic dermatitis where Th2 cell recruitment and activity are heightened. In asthma increased CCL17 levels exacerbate inflammatory response in the airways. In atopic dermatitis CCL17 presence corresponds with the intensity of the skin inflammation. These conditions are linked to the overactivity of Th2 cytokines such as IL-4 (H4L3) which collaborate with CCL17 to drive allergic responses.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Chemokine, which displays chemotactic activity for T lymphocytes, preferentially Th2 cells, but not monocytes or granulocytes. Therefore plays an important role in a wide range of inflammatory and immunological processes (PubMed : 8702936, PubMed : 9169480). Acts by binding to CCR4 at T-cell surface (PubMed : 10540332, PubMed : 9169480). Mediates GM-CSF/CSF2-driven pain and inflammation (PubMed : 27525438). In the brain, required to maintain the typical, highly branched morphology of hippocampal microglia under homeostatic conditions. May be important for the appropriate adaptation of microglial morphology and synaptic plasticity to acute lipopolysaccharide (LPS)-induced neuroinflammation (By similarity). Plays a role in wound healing, mainly by inducing fibroblast migration into the wound (By similarity).
See full target information CCL17

대체 명칭 보기

SCYA17, TARC, CCL17, C-C motif chemokine 17, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine
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Product promise

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