Anti-Tau (acetyl K311) antibody [EPR29545-504]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tau (acetyl K311) antibody [EPR29545-504] (AB324728)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Tau (acetyl K311) with ab324728 at 1/1000 (0.512 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ab150081 1000 2ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells co-transfected with a human 2N4R Tau (WT) expression vector containing a myc-His-tag® and a human CBP HAT domain expression vector containing a myc-His-tag® and no staining in 293T cells co-transfected with a human 2N4R Tau (K311R) expression vector containing a myc-His-tag® and a human CBP HAT domain expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
A : 293T cells co-transfected with a human 2N4R Tau (WT) expression vector containing a myc-His-tag® and a human CBP HAT domain expression vector containing a myc-His-tag®.
B : 293T cells co-transfected with a human 2N4R Tau (K311R) expression vector containing a myc-His-tag® and a human CBP HAT domain expression vector containing a myc-His-tag®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ab150081 1000 2ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tau (acetyl K311) antibody [EPR29545-504] (AB324728)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Tau (acetyl K311) with ab324728 at 1/1000 (0.512 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ab150081 1000 2ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells co-transfected with a human 2N4R Tau (WT) expression vector containing a myc-His-tag® and a human CBP HAT domain expression vector containing a myc-His-tag® and no staining in 293T cells transfected with an empty vector containing a myc-His-tag® and 293T cells transfected with a human 2N4R Tau (WT) expression vector containing a myc-His-tag® and 293T cells transfected with a human 2N4R Tau (K311R) expression vector containing a myc-His-tag® and 293T cells co-transfected with a human 2N4R Tau (K311R) expression vector containing a myc-His-tag® and a human CBP HAT domain expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
A : 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®
B : 293T cells transfected with a human 2N4R Tau (WT) expression vector containing a myc-His-tag®
C : 293T cells co-transfected with a human 2N4R Tau (WT) expression vector containing a myc-His-tag® and a human CBP HAT domain expression vector containing a myc-His-tag®.
D : 293T cells transfected with a human 2N4R Tau (K311R) expression vector containing a myc-His-tag®
E : 293T cells co-transfected with a human 2N4R Tau (K311R) expression vector containing a myc-His-tag® and a human CBP HAT domain expression vector containing a myc-His-tag®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ab150081 1000 2ug/ml dilution.

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Tau (acetyl K311) antibody [EPR29545-504] (AB324728)

Indirect ELISA analysis of ab324728 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Tau (acetyl K311) peptide;Tau non-acetyl peptide;MAP2 (acetyl K1697) peptide.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with human MAP2 (acetyl K1697) peptide by ELISA.

• WB

Supplier Data

Western blot - Anti-Tau (acetyl K311) antibody [EPR29545-504] (AB324728)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Tau antibody [EP2456Y] - (ab76128) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Tau (acetyl K311) antibody [EPR29545-504] (ab324728) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag, whole cell lysate at 4 µg

Lane 2:

293T cells transfected with a human 2N4R Tau (WT) expression vector containing a myc-His-tag®, whole cell lysate at 4 µg

Lane 3:

293T cells co-transfected with a human 2N4R Tau (WT) expression vector containing a myc-His-tag® and a human CBP HAT domain expression vector containing a myc-His-tag® whole cell lysate at 4 µg

Lane 4:

293T cells transfected with a human 2N4R Tau (K311R) expression vector containing a myc-His-tag®, whole cell lysate at 4 µg

Lane 5:

293T cells co-transfected with a human 2N4R Tau (K311R) expression vector containing a myc-His-tag® and a human CBP HAT domain expression vector containing a myc-His-tag®, whole cell lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 70 kDa,36 kDa

false

Exposure time: 180s