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AB315892

Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] - BSA and Azide free

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Rabbit Recombinant Monoclonal TAU nitrosyl Y29 antibody. Carrier free. Suitable for WB, I-ELISA, Dot and reacts with Transfected cell lysate - Human, Synthetic peptide - Human samples.

대체 명칭 보기

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

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Indirect ELISA - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] - BSA and Azide free (AB315892)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] - BSA and Azide free (AB315892)

This data was developed using ab315891, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab315891 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Tau (nitrosylated Y29) peptide a,Tau (nitrosylated Y29) peptide b,Tau non-nitrosylated Y29 peptide c,Tau (phospho Y29) peptide d,Tau (nitrosylated Y18) peptide e,Tau (nitrosylated Y18) peptide f.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] - BSA and Azide free (AB315892)
  • WB

Supplier Data

Western blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] - BSA and Azide free (AB315892)

This data was developed using ab315891, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Tau antibody - Total protein control (ab254256) staining at 1/1000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (<a href='/ko/products/primary-antibodies/tau-nitrosyl-y29-antibody-epr27841-79-ab315891'>ab315891</a>) at 1/1000 dilution

Lane 1:

Untreated 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with an empty vector containing a His-tag were treated with 3mM peroxynitrite for 1 hour, whole cell lysate at 20 µg

Lane 3:

Untreated 293T cells transfected with a human Tau 2N4R expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human Tau 2N4R expression vector containing a His-tag were treated with 3mM peroxynitrite for 1 hour, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20-75 kDa,36 kDa

false

Exposure time: 180s

Dot Blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] - BSA and Azide free (AB315892)
  • Dot

Supplier Data

Dot Blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] - BSA and Azide free (AB315892)

This data was developed using ab315891, the same antibody clone in a different buffer formulation.

Dot blot analysis of Tau (nitrosyl Y29) using ab315891 at 1 : 1000 (0.518 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (<a href='/ko/products/primary-antibodies/tau-nitrosyl-y29-antibody-epr27841-79-ab315891'>ab315891</a>) at 1/1000 dilution

Lane 1:

Tau (nitrosylated Y29) peptide a

Lane 2:

Tau (nitrosylated Y29) peptide b

Lane 3:

Tau non-nitrosylated Y29 peptide c

Lane 4:

Tau (phospho Y29) peptide d

Lane 5:

Tau (nitrosylated Y18) peptide e

Lane 6:

Tau (nitrosylated Y18) peptide f

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Unconjugated

    Anti-Tau (nitrosyl Y29) antibody [EPR27841-79]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27841-79

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Dot, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Reagents, Controls & Accessories

AB7475

Normal Donkey Serum

Immunohistochemistry (PFA perfusion fixed frozen sections) - Normal Donkey Serum (AB7475)

  • 0
  • 0
Reagents, Controls & Accessories

AB108410

DRAQ5™

Immunocytochemistry/ Immunofluorescence - DRAQ5™ (AB108410)

  • 5
  • 5
Reagents, Controls & Accessories

AB7477

Normal Chicken Serum (sterile)

  • 0
  • 0
Reagents, Controls & Accessories

AB138579

Normal Donkey serum (sterile)

  • 0
  • 0
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Reactivity 정보

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Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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제품 세부 정보

ab315892 is the carrier-free version of ab315891.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT nitrosyl Y29
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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