AB170892

Anti-Tau (phospho S214) antibody [EPR1884(2)]

Name: Anti-Tau (phospho S214) antibody [EPR1884(2)]
Brand: Abcam Limited
SKU: AB170892
Rating: 5 (1 reviews)

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(23 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal TAU phospho S214 antibody. Suitable for IHC-P, Dot, WB, I-ELISA and reacts with Mouse, Human, Synthetic peptide, Synthetic peptide - Human samples. Cited in 23 publications.

11 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

ab170892 showing -ve staining in Human normal spleen tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human AD cerebral cortex tissue labelling Tau with ab170892 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.

Positive staining on human AD cerebral cortex without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).

The section was incubated with ab170892 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

ab170892 showing -ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Tau (phospho S214) with ab170892 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

ab170892 showing -ve staining in Human cervical carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Indirect ELISA - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

I-ELISA

Lab

Indirect ELISA - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Indirect ELISA analysis of ab170892 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen : Tau (phospho T212, phospho S214) phospho peptide, Tau (phospho T212) phospho peptide, Tau (phospho S214) phospho peptide, Tau non-phospho peptide.

Antigen concentration : 1000 ng/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

ab170892 showing +ve staining in Mouse brain tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892) at 1/1000 dilution

Lane 1:

C57 mouse cerebral cortex whole cell lysates. at 15 µg

Lane 2:

C57 mouse cerebral cortex whole cell lysates. The membrane was incubated with phosphatase. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 50-70 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

All lanes:

Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysates untreated at 10 µg

Lane 2:

SH-SY5Y cell lysates treated with Okadic acid + Calyculin A. at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 78 kDa

false

Lab

Dot Blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Dot blot analysis of Tau (phospho S214) phospho peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labeling Tau (phospho S214) with ab170892 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

CiteAb

Immunohistochemistry - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Tau (phospho S214) Immunohistochemistry using Anti-Tau (phospho S214) antibody [EPR1884(2)], ab170892. Publication image from Fujita, K. et al., 2018, Nat Commun, PubMed : 29382817.

Mislocalization of human pSer214 to spine. a Immunohistochemistry of pSer214-tau, which corresponds to mouse pSer203-tau, in human sporadic FTLD-TDP autopsy samples revealed similar mislocalization of specific types of tau to PSD95-positive spines (left images). Averages and s.e.m. are shown. Five independent cases were examined, and the averaged values of 10 visual fields (40 × 40 μm) in each case were used for quantitative analysis (right graphs). P-values were determined by Student’s t-test (N = 5). **p < 0.01. b Pathological diagnosis of patients was based on the presence of TDP43. Findings from each case are shown. p-values are shown in Supplementary data 1

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1884(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Dot, WB, IHC-P, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "62.5 ng/mL", "IELISA-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT pS214

대체 명칭 보기

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

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제품이 사용된 논문 (23)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 8:1139 PubMed40745206

2025

Stoichiometric 14-3-3ζ binding promotes phospho-Tau microtubule dissociation and reduces aggregation and condensation.

Applications

Unspecified application

Species

Unspecified reactive species

Janine Hochmair,Maxime C M van den Oetelaar,Leandre Ravatt,Lisa Diez,Lenne J M Lemmens,Renata Ponce-Lina,Rithika Sankar,Maximilian Franck,Gesa Nolte,Ekaterina Semenova,Satabdee Mohapatra,Christian Ottmann,Luc Brunsveld,Susanne Wegmann

Journal of extracellular vesicles 13:e12439 PubMed38647111

2024

Neuropathogenic role of astrocyte-derived extracellular vesicles in HIV-associated neurocognitive disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Divya T Chemparathy,Sudipta Ray,Chase Ochs,Natasha Ferguson,Dinesh Y Gawande,Shashank M Dravid,Shannon Callen,Susmita Sil,Shilpa Buch

Molecular medicine (Cambridge, Mass.) 30:4 PubMed38172666

2024

Key roles of autophagosome/endosome maturation mediated by Syntaxin17 in methamphetamine-induced neuronal damage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Wang,Miaoyang Hu,Jingrong Chen,Xinyu Lou,Hongchao Zhang,Muhan Li,Jie Cheng,Tengfei Ma,Jianping Xiong,Rong Gao,Xufeng Chen,Jun Wang

eNeuro 10: PubMed36898832

2023

Characterization of the Tau Interactome in Human Brain Reveals Isoform-Dependent Interaction with 14-3-3 Family Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan K Betters,Emma Luhmann,Amy C Gottschalk,Zhen Xu,Mallory R Shin,Christopher P Ptak,Kimberly L Fiock,Lilliana C Radoshevich,Marco M Hefti

Science advances 8:eadd2577 PubMed36459557

2022

Treatment of epilepsy using a targeted p38γ kinase gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolle Morey,Magdalena Przybyla,Julia van der Hoven,Yazi D Ke,Fabien Delerue,Janet van Eersel,Lars M Ittner

Experimental and therapeutic medicine 23:426 PubMed35607377

2022

Effect of ginkgolide K on calcium channel activity in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbin Liu,Qinyun Li,Xiaodan Zhang,Yun Shi,Jinyi Li

Acta pharmacologica Sinica 43:1916-1927 PubMed34893682

2021

PINK1 overexpression prevents forskolin-induced tau hyperphosphorylation and oxidative stress in a rat model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Juan Wang,Lin Qi,Ya-Fang Cheng,Xue-Fei Ji,Tian-Yan Chi,Peng Liu,Li-Bo Zou

Neurobiology of aging 109:52-63 PubMed34655981

2021

Pathological tau and reactive astrogliosis are associated with distinct functional deficits in a mouse model of tauopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Henika Patel,Pablo Martinez,Abigail Perkins,Xavier Taylor,Nur Jury,David McKinzie,Cristian A Lasagna-Reeves

Communications biology 4:1175 PubMed34635772

2021

HMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer's disease pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Hikari Tanaka,Kanoh Kondo,Kyota Fujita,Hidenori Homma,Kazuhiko Tagawa,Xiaocen Jin,Meihua Jin,Yuki Yoshioka,Sumire Takayama,Hitomi Masuda,Rie Tokuyama,Yukoh Nakazaki,Takashi Saito,Takaomi Saido,Shigeo Murayama,Teikichi Ikura,Nobutoshi Ito,Yu Yamamori,Kentaro Tomii,Marco E Bianchi,Hitoshi Okazawa

Communications biology 4:961 PubMed34385591

2021

Prediction and verification of the AD-FTLD common pathomechanism based on dynamic molecular network analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Meihua Jin,Xiaocen Jin,Hidenori Homma,Kyota Fujita,Hikari Tanaka,Shigeo Murayama,Hiroyasu Akatsu,Kazuhiko Tagawa,Hitoshi Okazawa

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tau (phospho S214) antibody [EPR1884(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Indirect ELISA - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Dot Blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

Immunohistochemistry - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (23)

Product promise