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AB196365

Anti-Tau (phospho S422) antibody [EPR2866] - BSA and Azide free

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(1 출판물)

Rabbit Recombinant Monoclonal TAU phospho S422 antibody. Carrier free. Suitable for Dot, WB, Agg and reacts with Synthetic peptide, Human samples. Cited in 1 publication.

대체 명칭 보기

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

2 이미지
Western blot - Anti-Tau (phospho S422) antibody [EPR2866] - BSA and Azide free (AB196365)
  • WB

Lab

Western blot - Anti-Tau (phospho S422) antibody [EPR2866] - BSA and Azide free (AB196365)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79415).

Blocking and dilution buffer : 5% NFDM/TBST.

Western blot for ab205606 on the right hand side used as a control for flag tag used.

All lanes:

Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (<a href='/ko/products/primary-antibodies/tau-phospho-s422-antibody-epr2866-ab79415'>ab79415</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a flag tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Tau expression vector containing a flag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human MAP2 expression vector containing a flag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human MAP4 expression vector containing a flag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50-100 kDa

false

Exposure time: 1s

Dot Blot - Anti-Tau (phospho S422) antibody [EPR2866] - BSA and Azide free (AB196365)
  • Dot

Unknown

Dot Blot - Anti-Tau (phospho S422) antibody [EPR2866] - BSA and Azide free (AB196365)

Dot blot analysis of Tau (pS422) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS422) with ab79415 at a dilution of 1/1000. ab97051 (Peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79415).

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2866

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Agg, Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8&#46;

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대체품

Primary Antibodies

AB92682

Anti-Tau (phospho Ser673) antibody [EPR2603]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Tau (phospho Ser673) antibody [EPR2603] (AB92682)

  • 0
  • 0
Primary Antibodies

AB109390

Anti-Tau (phospho S396) antibody [EPR2731]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (AB109390)

  • 4
  • 14
Primary Antibodies

AB151559

Anti-Tau (phospho T231) antibody [EPR2488]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559)

  • 5
  • 6
Primary Antibodies

AB32057

Anti-Tau (phospho S396) antibody [E178]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (AB32057)

  • 5
  • 5
Primary Antibodies

AB170892

Anti-Tau (phospho S214) antibody [EPR1884(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size|Trial Size|Trial Size|Trial Size|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

  • 5
  • 1
Primary Antibodies

AB92676

Anti-Tau (phospho S404) antibody [EPR2605]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (AB92676)

  • 5
  • 4
Primary Antibodies

AB93726

Anti-Tau antibody [SP70]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [SP70] (AB93726)

  • 5
  • 4

보완 제품

Primary Antibodies

AB109390

Anti-Tau (phospho S396) antibody [EPR2731]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (AB109390)

  • 4
  • 14
Primary Antibodies

AB278054

Anti-GFAP antibody [RM1003] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [RM1003] - Astrocyte Marker (AB278054)

  • 0
  • 0
Primary Antibodies

AB76128

Anti-Tau antibody [EP2456Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Western blot - Anti-Tau antibody [EP2456Y] (AB76128)

  • 5
  • 1
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Agg" : {"fullname" : "Agglutination", "shortname":"Agg"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "Agg-species-checked": "guaranteed", "Agg-species-dilution-info": "", "Agg-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Agg-species-checked": "notRecommended", "Agg-species-dilution-info": "", "Agg-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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제품 세부 정보

ab196365 is the carrier-free version of ab79415.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT phospho S422
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

iScience 28:112036 PubMed40115028

2025

Rocking during sleep reduces motor deficits and beta-amyloid levels in an Alzheimer's mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Luyan Zhang,Letizia Santoni,Nam Anh Ngo,Reyila Simayi,Eleonora Ficiará,Luisa de Vivo,Michele Bellesi
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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