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AB223192

Anti-Tau (phospho T181) antibody [EPR22064]

5

(4 리뷰들)

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(1 출판물)

Rabbit Recombinant Monoclonal TAU phospho T181 antibody. Suitable for IP, Dot, WB, I-ELISA and reacts with Human, Synthetic peptide, Synthetic peptide - Human samples. Cited in 1 publication.
4 이미지
Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)
  • IP

Unknown

Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)

Tau (phospho T181 was immunoprecipitated from 0.35 mg of human brain lysate with ab223192 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223192 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human brain lysate 10 μg (Input).
Lane 2 : ab223192 IP in Human brain lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab223192 in Human brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST
Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] (ab223192) at 1/500 dilution

Predicted band size: 79 kDa

Observed band size: 60 kDa

false

Indirect ELISA - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)
  • I-ELISA

Lab

Indirect ELISA - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)

Indirect ELISA analysis of ab223192 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Human MAPT(phospho T181) peptide, Human MAPT non-phospho peptide

Antigen concentration : 1000 ng/ml.

Western blot - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)
  • WB

Unknown

Western blot - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)

Blocking and dilution buffer : 5% NFDM/TBST.

WB membrane in Lane 2 was treated with alkaline phosphatase for 1h at 37°C.

All lanes:

Western blot - Anti-Tau (phospho T181) antibody [EPR22064] (ab223192) at 1/1000 dilution

Lane 1:

Human brain lysate at 10 µg

Lane 2:

Human brain lysate (WB membrane treated with alkaline phosphatase) at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 79 kDa

Observed band size: 60 kDa

false

Exposure time: 3min

Dot Blot - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)
  • Dot

Unknown

Dot Blot - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)

Dot blot analysis of Tau (phospho T181) labeled with ab223192 at 1/1000 dilution.

Lane 1 : Tau (phospho T181) peptide.
Lane 2 : Tau non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22064

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, IP, WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>The specificity of this antibody refers to P10636-8&#46;</p>

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대체품

Primary Antibodies

AB92682

Anti-Tau (phospho Ser356) antibody [EPR2603]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Tau (phospho Ser356) antibody [EPR2603] (AB92682)

  • 0
  • 0
Primary Antibodies

AB109390

Anti-Tau (phospho S396) antibody [EPR2731]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (AB109390)

  • 4
  • 14
Primary Antibodies

AB151559

Anti-Tau (phospho T231) antibody [EPR2488]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559)

  • 5
  • 6
Primary Antibodies

AB32057

Anti-Tau (phospho S396) antibody [E178]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (AB32057)

  • 5
  • 5
Primary Antibodies

AB170892

Anti-Tau (phospho S214) antibody [EPR1884(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size|Trial Size|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

  • 5
  • 1
Primary Antibodies

AB92676

Anti-Tau (phospho S404) antibody [EPR2605]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (AB92676)

  • 5
  • 4
Primary Antibodies

AB93726

Anti-Tau antibody [SP70]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [SP70] (AB93726)

  • 5
  • 4

보완 제품

Primary Antibodies

AB170892

Anti-Tau (phospho S214) antibody [EPR1884(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

  • 5
  • 1
Primary Antibodies

AB151559

Anti-Tau (phospho T231) antibody [EPR2488]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559)

  • 5
  • 6
Primary Antibodies

AB32057

Anti-Tau (phospho S396) antibody [E178]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (AB32057)

  • 5
  • 5
Primary Antibodies

AB291080

Anti-Tau (phospho T217) antibody [EPR24654-110]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "62.5 ng/mL", "IELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT pT181

대체 명칭 보기

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature 644:1058-1068 PubMed40659786

2025

Microbiota-driven antitumour immunity mediated by dendritic cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Yi-Tzu Lin,Shota Fukuoka,Shohei Koyama,Daisuke Motooka,Dieter M Tourlousse,Yuko Shigeno,Yuki Matsumoto,Hiroyuki Yamano,Kazutoshi Murotomi,Hideyuki Tamaki,Takuma Irie,Eri Sugiyama,Shogo Kumagai,Kota Itahashi,Tokiyoshi Tanegashima,Kaori Fujimaki,Sachiko Ito,Mariko Shindo,Takahiro Tsuji,Hiroaki Wake,Keisuke Watanabe,Yuka Maeda,Tomohiro Enokida,Makoto Tahara,Riu Yamashita,Takao Fujisawa,Motoo Nomura,Akihito Kawazoe,Koichi Goto,Toshihiko Doi,Kohei Shitara,Hiroyuki Mano,Yuji Sekiguchi,Shota Nakamura,Yoshimi Benno,Hiroyoshi Nishikawa
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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