AB4842

Anti-Tau (phospho T212) antibody

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(7 제품이 사용된 논문 )

Rabbit Polyclonal TAU phospho T212 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MAPT pT212.

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Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho T212) antibody (AB4842)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho T212) antibody (AB4842)

SHSY5Y cells stained for Tau (green) using ab4842 at 1μg/mL in ICC/IF. Followed by Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at 1/400 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing nuclear and cytoplasmic localization. Panel e is a no primary antibody control.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

Immunogen

Synthetic Peptide within Human MAPT pT212. The exact immunogen used to generate this antibody is proprietary information.

Specificity

Phosphorylation site-specific antibody selective for the phosphorylated form of human tau containing a phosphate on threonine 212. <p>The specificity of this antibody refers to P10636-8.</p>

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대체품

Primary Antibodies

AB92682

Anti-Tau (phospho Ser356) antibody [EPR2603]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Tau (phospho Ser356) antibody [EPR2603] (AB92682)

0
0

Primary Antibodies

AB109390

Anti-Tau (phospho S396) antibody [EPR2731]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (AB109390)

4
14

Primary Antibodies

AB151559

Anti-Tau (phospho T231) antibody [EPR2488]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559)

5
6

Primary Antibodies

AB32057

Anti-Tau (phospho S396) antibody [E178]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (AB32057)

5
5

Primary Antibodies

AB170892

Anti-Tau (phospho S214) antibody [EPR1884(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size|Trial Size|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

5
1

Primary Antibodies

AB92676

Anti-Tau (phospho S404) antibody [EPR2605]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (AB92676)

5
4

Primary Antibodies

AB93726

Anti-Tau antibody [SP70]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [SP70] (AB93726)

5
4

보완 제품

Primary Antibodies

AB76128

Anti-Tau antibody [EP2456Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Western blot - Anti-Tau antibody [EP2456Y] (AB76128)

5
1

Primary Antibodies

AB151559

Anti-Tau (phospho T231) antibody [EPR2488]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559)

5
6

Primary Antibodies

AB4749

Anti-Tau (phospho S199) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S199) antibody (AB4749)

5
1

Primary Antibodies

AB4841

Anti-Tau (phospho T205) antibody

Flow Cytometry - Anti-Tau (phospho T205) antibody (AB4841)

0
0

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide that is phosphorylated at threonine 212.

보관 버퍼

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT pT212

대체 명칭 보기

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of medicinal chemistry 66:15648-15670 PubMed38051674

2023

Chemical, Biochemical, Cellular, and Physiological Characterization of Leucettinib-21, a Down Syndrome and Alzheimer's Disease Drug Candidate.

Applications

Unspecified application

Species

Unspecified reactive species

Mattias F Lindberg,Emmanuel Deau,Frédéric Miege,Marie Greverie,Didier Roche,Nicolas George,Pascal George,Laura Merlet,Julie Gavard,Sander J T Brugman,Edwin Aret,Paul Tinnemans,René de Gelder,Jan Sadownik,Eva Verhofstad,Dennis Sleegers,Sara Santangelo,Julien Dairou,Álvaro Fernandez-Blanco,Mara Dierssen,Andreas Krämer,Stefan Knapp,Laurent Meijer

Science advances 8:eabl8809 PubMed35857446

2022

Alzheimer's disease: Ablating single master site abolishes tau hyperphosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Kristie Stefanoska,Mehul Gajwani,Amanda R P Tan,Holly I Ahel,Prita R Asih,Alexander Volkerling,Anne Poljak,Arne Ittner

Frontiers in physiology 10:110 PubMed30837891

2019

Increased Ratio of Global -GlcNAcylation to Tau Phosphorylation at Thr212 Site Is Associated With Better Memory Function in Patients With Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Huang,Sai Tian,Jing Han,Rongrong Cai,Hongyan Lin,Dan Guo,Jiaqi Wang,Shaohua Wang

Scientific reports 8:17702 PubMed30531974

2018

Phosphorylation of nuclear Tau is modulated by distinct cellular pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgio Ulrich,Agnese Salvadè,Paul Boersema,Tito Calì,Chiara Foglieni,Martina Sola,Paola Picotti,Stéphanie Papin,Paolo Paganetti

The Journal of cell biology 216:3161-3178 PubMed28877993

2017

Stabilization of dynamic microtubules by mDia1 drives Tau-dependent Aβ synaptotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyi Qu,Feng Ning Yuan,Carlo Corona,Silvia Pasini,Maria Elena Pero,Gregg G Gundersen,Michael L Shelanski,Francesca Bartolini

Journal of cell science 122:2424-35 PubMed19549690

2009

Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons.

Applications

WB

Species

Unspecified reactive species

Timothy M E Scales,Shen Lin,Michaela Kraus,Robert G Goold,Phillip R Gordon-Weeks

Journal of Alzheimer's disease : JAD 16:341-50 PubMed19221424

2009

Alzheimer's disease-specific tau phosphorylation is induced by herpes simplex virus type 1.

Applications

ICC/IF

Species

Human

Matthew A Wozniak,Alison L Frost,Ruth F Itzhaki

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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