AB291080

Anti-Tau (phospho T217) antibody [EPR24654-110]

Anti-Tau (phospho T217) 항체 [EPR24654-110]

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) is a rabbit monoclonal antibody detecting Tau in Western Blot, IP, ICC/IF, ELISA, Dot Blot, and IHC-p. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Tau (phospho T217) with ab291080 at 1/25000 dilution (0.02 μg/ml).

Negative control : no staining on human stomach.

The section was incubated with ab291080 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument using LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody, ready to use.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemical analysis of paraffin-embedded Human normal brain tissue labeling Tau (phospho T217) with ab291080 at 1/25000 dilution (0.02 μg/ml).

Negative control : no staining on human normal brain.

The section was incubated with ab291080 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument using LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody, ready to use.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemical analysis of paraffin-embedded Human Alzheimer's disease brain tissue labeling Tau (phospho T217) with ab291080 at 1/25000 dilution (0.02 μg/ml).

Positive staining on human Alzheimer's disease brain.

The section was incubated with primary antibody ab291080 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

The secondary antibody used was LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Sodium-Citrate buffer (pH 6.0, Epitope Retrieval Solution1) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Tau (phospho T217) with ab291080 at 1/25000 dilution (0.02 μg/ml).

Negative control : no staining on human skeletal muscle.

The section was incubated with ab291080 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument using LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody, ready to use.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

I-ELISA

Lab

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Antigen human Tau (phospho T217) peptide and a control human Tau non-phospho peptide were at 1000 ng/ml. ab291080 was used at 1000-0 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1/2500 dilution.

Lab

Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200,000 dilution.

In Western blot, Anti-Tau antibody [EP2456Y] (ab76128) (1/1000) (55KDa)

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid femtogram range.

All lanes:

Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) at 1/1000 dilution

Lane 1:

Rat P0 brain (Untreated membrane) at 20 µg

Lane 2:

Rat P0 brain (Phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50-80 kDa,36 kDa,55 kDa

true

Exposure time: 180s

Lab

Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) at 1/1000 dilution

Lane 1:

Tau (phospho T217) peptide

Lane 2:

Tau (phospho T220) peptide

Lane 3:

Tau non-phospho T217 peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24654-110

Isotype

IgG

Carrier free

Reacts with

Human, Rat

Applications

ICC/IF, I-ELISA, Dot, IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide - Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

What is this antibody validated in?
Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Dot Blot in Human samples.

What is the molecular weight of Tau?
Anti-Tau (phospho T217) [EPR24654-110] (ab291080) specifically detects a band for Tau (UniProt: P10636) at a molecular weight of 46kDa.

Other related products
We have a range of other formats of antibody clone [EPR24654-110] also available for your convenience: ab291080, Carrier free - ab291104

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3Β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT pT217

대체 명칭 보기

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Alzheimer's research & therapy 16:267 PubMed39702316

2024

Structural and functional alterations of neurons derived from sporadic Alzheimer's disease hiPSCs are associated with downregulation of the LIMK1-cofilin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Raimondo Sollazzo,Domenica Donatella Li Puma,Giuseppe Aceto,Fabiola Paciello,Claudia Colussi,Maria Gabriella Vita,Guido Maria Giuffrè,Francesco Pastore,Alessia Casamassa,Jessica Rosati,Agnese Novelli,Sabrina Maietta,Francesco Danilo Tiziano,Camillo Marra,Cristian Ripoli,Claudio Grassi

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tau (phospho T217) antibody [EPR24654-110]

Anti-Tau (phospho T217) 항체 [EPR24654-110]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise