Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free

• IHC-Fr

AbReview60326****

Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (AB239000)

ab183032 staining TBR1 in Human cerebral organoid tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeabilized with 0.1% Triton X, blocked with 5% serum for 1 hour at room temperature and antigen retrieval was by heat mediation in Dako target retrieval. The sample was incubated with primary antibody (1 : 300 dilution) at 4^oC for 12 hours.  An Alexa Fluor® 488 conjugate goat polyclonal (1/200) was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183032).

This image is courtesy of an Abreview submitted by Daniel Berg.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (AB239000)

Immunohistochemistry of paraffin embedded Mouse E14 cerebral cortex labelling TBR1 with ab183032 at 0.011μg/ml.  Antigen retrieval was heat mediated using Tris/EDTA buffer, pH 9.0 (ab93684). Secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP). Counter stained using Hematoxylin. Nuclear staining on cerebral cortex of mouse E14 [PMID : 27848932].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183032).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (AB239000)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling tbr1 with ab183032 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 antibody [HM-2] at 1/100 dilution and ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594 at 1/1000 (2μg/ml) was used as a MAP2 counterstain. The Nuclear counterstain was DAPI (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183032).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (AB239000)

Immunohistochemistry of paraffin embedded Rat E14 cerebral cortex labelling TBR1 with ab183032 at 0.011μg/ml. Antigen retrieval was heat mediated using Tris/EDTA buffer, pH 9.0 (ab93684). Secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP). Counter stained using Hematoxylin. Nuclear staining on cerebral cortex of rat E14 [PMID : 27848932].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183032).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (AB239000)

ab183032 staining TBR-1 in Rat E14 cerebral cortex sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with  4% paraformaldehyde and permeabilized with 0.2% TritonX-100.  AlexaFluor®488 Goat anti-Rabbit (2 μg/ml) (ab150077) was used as the secondary antibody. Counter stained using DAPI. Nuclear staining on cerebral cortex of rat E14 [PMID : 27848932].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183032).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (AB239000)

ab183032 staining TBR-1 in Mouse E14 cerebral cortex sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with  4% paraformaldehyde and permeabilized with 0.2% TritonX-100.  AlexaFluor®488 Goat anti-Rabbit (2 μg/ml) (ab150077) was used as the secondary antibody. Counter stained using DAPI. Nuclear staining on cerebral cortex of mouse E14 [PMID : 27848932].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183032).