AB281835

Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TBX5 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.

대체 명칭 보기

T-box transcription factor TBX5, T-box protein 5, Tbx5

1 이미지

Lab

Western blot - Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free (AB281835)

This data was developed using 259980, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : mouse embryo/fetal liver (PIMID : 16894625).

Exposure time : 70 seconds

All lanes:

Western blot - Anti-TBX5 antibody [EPR23798-102] (<a href='/ko/products/primary-antibodies/tbx5-antibody-epr23798-102-ab259980'>ab259980</a>) at 1/1000 dilution

Lane 1:

Mouse P0 heart tissue lysate at 20 µg

Lane 2:

Mouse P0 liver tissue lysate at 20 µg

Lane 3:

Mouse E18 heart tissue lysate at 20 µg

Lane 4:

Mouse E18 liver tissue lysate at 20 µg

Lane 5:

Mouse E17 heart tissue lysate at 20 µg

Lane 6:

Mouse E17 liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 58 kDa

Observed band size: 57 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23798-102

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab281835 is the carrier-free version of ab259980.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TBX5 also known as T-box transcription factor 5 is a protein that acts as a transcription factor. It has a mass of approximately 53 kDa. TBX5 is expressed significantly in the heart and limb tissues highlighting its importance in these areas. The protein contains a T-box domain that binds to specific DNA sequences allowing it to regulate the transcription of genes involved in developmental processes. TBX5 plays a critical role during embryogenesis particularly affecting cardiac and limb development.

Biological function summary

The function of TBX5 extends to various cellular processes. It is involved in forming the cardiac septum and limb bud acting through its role as part of the transcriptional program. TBX5 doesn't typically form a complex with other proteins but interacts with partners like GATA4 to influence gene expression. These interactions enable TBX5 to activate or repress target genes necessary for organ and limb morphogenesis making it fundamental in developmental biology.

Pathways

TBX5 operates within the cardiac development and limb differentiation pathways. It interacts with other critical factors such as NKX2-5 and TBX3 to coordinate the differentiation and proliferation processes essential for forming functional heart and limb structures. In heart development TBX5 and NKX2-5 synergistically work to activate cardiac-specific genes influencing the patterning and structural formation of the heart. The protein's involvement in limb differentiation emphasizes its dual role in these important developmental pathways.

TBX5 has significant associations with conditions like Holt-Oram syndrome and congenital heart defects. Holt-Oram syndrome a disorder arising from mutations in the TBX5 gene is characterized by limb abnormalities and cardiac anomalies illustrating the protein's role in heart and limb development. TBX5 is also linked to cardiac arrhythmias with its molecular pathways involving proteins like NKX2-5. Mutations leading to TBX5 dysfunction impact these pathways resulting in developmental malformations and contributing to complex congenital conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation. Binds to the core DNA motif of NPPA promoter.

See full target information Tbx5

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free

Western blot - Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free (AB281835)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise