Anti-TCF7L2 antibody [2C2]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 antibody [2C2] (AB326709)

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TCF7L2 Monoclonal Antibody, Unconjugated (ab326709) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-TCF7L2 antibody [2C2] (AB326709)

HepG2 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TCF7L2 Monoclonal Antibody (ab326709)at 1 : 50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 antibody [2C2] (AB326709)

Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TCF7L2 Monoclonal Antibody, Unconjugated (ab326709) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 antibody [2C2] (AB326709)

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TCF7L2 Monoclonal Antibody, Unconjugated (ab326709) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 antibody [2C2] (AB326709)

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TCF7L2 Monoclonal Antibody, Unconjugated (ab326709) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 antibody [2C2] (AB326709)

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TCF7L2 Monoclonal Antibody, Unconjugated (ab326709) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• WB

Supplier Data

Western blot - Anti-TCF7L2 antibody [2C2] (AB326709)

25 ug total protein per lane of various lysates (see on figure) probed with TCF7L2 monoclonal antibody, unconjugated (ab326709) at 1 : 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

All lanes:

Western blot - Anti-TCF7L2 antibody [2C2] (ab326709) at 1/1000 dilution

Lane 1:

HT-29 at 25 µg

Lane 2:

K562 at 25 µg

false