AB133568

Anti-TCTP antibody [EPR5540]

Name: Anti-TCTP antibody [EPR5540]
Brand: Abcam Limited
SKU: AB133568
Rating: 5 (3 reviews)

RabMAb
Recombinant
각 태그의 뜻

(3 리뷰들)

(12 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal TCTP antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

대체 명칭 보기

Translationally-controlled tumor protein, TCTP, Fortilin, Histamine-releasing factor, p23, HRF, TPT1

3 이미지

Flow Cytometry (Intracellular) - Anti-TCTP antibody [EPR5540] (AB133568)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TCTP antibody [EPR5540] (AB133568)

Overlay histogram showing A431 cells stained with ab133568 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133568, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Immunocytochemistry/ Immunofluorescence - Anti-TCTP antibody [EPR5540] (AB133568)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCTP antibody [EPR5540] (AB133568)

Immunofluorescence analysis of A431 cells staining TCTP (green) using ab133568 at a 1/250 dilution. Cell nuclei are shown in blue.

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Western blot - Anti-TCTP antibody [EPR5540] (AB133568)

All lanes:

Western blot - Anti-TCTP antibody [EPR5540] (ab133568) at 1/10000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 20 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5540

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TCTP also known as Translationally Controlled Tumor Protein or histamine-releasing factor (HRF) is a multifunctional protein with a molecular mass of approximately 19 kDa. It is found in a variety of tissues and cell types including cytoplasm and extracellular environments. The protein engages in cellular processes such as cell growth development and apoptosis. TCTP regulates microtubule stability cell cycle progression and protein synthesis reflecting its broad mechanical roles within cells.

Biological function summary

In both animal and human cells TCTP acts as a prosurvival factor by inhibiting apoptosis. It binds with proteins like Bcl-2 and Bax playing a role in the regulation of cell death. Furthermore TCTP appears to mediate immune responses through its histamine-releasing factor activity. It interacts within protein complexes that manage cytoskeletal dynamics and stress responses contributing to cellular adaptability.

Pathways

The protein interacts in critical pathways such as the MAPK signaling pathway and the apoptosis pathway. In the MAPK pathway TCTP modulates cell growth and proliferation by interacting with proteins like ERK1/2. In the apoptosis pathway TCTP's interaction with Bcl-xL and Bax highlights its role in the fine-tuning of cell survival and death processes. These pathways highlight its participation in essential cellular functions and disease progression.

TCTP has implications in cancer and allergy. Altered expression and function of TCTP have been linked to tumor growth and resistance to cancer therapies often regulated through complex interactions with proteins like p53. In allergies its role in histamine release positions it as a mediator in allergic asthma and other hypersensitivity reactions. These associations emphasize the clinical relevance of TCTP and its potential as a therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Involved in calcium binding and microtubule stabilization (PubMed : 12167714, PubMed : 15162379, PubMed : 15958728). Acts as a negative regulator of TSC22D1-mediated apoptosis, via interaction with and destabilization of TSC22D1 protein (PubMed : 18325344).

See full target information Translationally-controlled tumor protein

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제품이 사용된 논문 (12)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 8:1040 PubMed40640354

2025

Fortilin deficiency induces anti-atherosclerotic phenotypes in macrophages and protects hypercholesterolemic mice against atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Nattaporn Wanachottrakul,Decha Pinkaew,Sandipan Mukherjee,Preedakorn Chunhacha,Mari Nakashima,Asa A Brockman,Uttariya Pal,Hasseri B Halim,Yuhong Wei,Lena Tanaka,Hanna Huynh,Kota Ramana,Shiyou Chen,Rebecca A Ihrie,Ken Fujise

Communications biology 8:1 PubMed39747445

2025

Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mari Nakashima,Decha Pinkaew,Uttariya Pal,Fei Miyao,Hanna Huynh,Lena Tanaka,Ken Fujise

Antioxidants (Basel, Switzerland) 12: PubMed37107185

2023

Deubiquitylase OTUD3 Mediates Endoplasmic Reticulum Stress through Regulating Fortilin Stability to Restrain Dopaminergic Neurons Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Chen,Xuejie Huan,Fengju Jia,Zhen Zhang,Mingxia Bi,Lin Fu,Xixun Du,Xi Chen,Chunling Yan,Qian Jiao,Hong Jiang

Communications biology 5:157 PubMed35197550

2022

Fortilin interacts with TGF-β1 and prevents TGF-β receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

Decha Pinkaew,Erik Martinez-Hackert,Wei Jia,Matthew D King,Fei Miao,Nicole R Enger,Runglawan Silakit,Kota Ramana,Shi-You Chen,Ken Fujise

Frontiers in molecular biosciences 8:686718 PubMed34589516

2021

Upregulation of Translationally Controlled Tumor Protein Is Associated With Cervical Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Zhu,Ji Ren,Dianqin Xu,Di Cheng,Wei Wang,Jie Ren,Ziwen Xiao,Hongmei Jiang,Yan Ding,Yujie Tan

Cells 9: PubMed32438775

2020

DHA Affects Microtubule Dynamics Through Reduction of Phospho-TCTP Levels and Enhances the Antiproliferative Effect of T-DM1 in Trastuzumab-Resistant HER2-Positive Breast Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia D'Amico,Ewa Krystyna Krasnowska,Isabella Manni,Gabriele Toietta,Silvia Baldari,Giulia Piaggio,Marco Ranalli,Alessandra Gambacurta,Claudio Vernieri,Flavio Di Giacinto,Francesca Bernassola,Filippo de Braud,Maria Lucibello

OncoTargets and therapy 12:1641-1653 PubMed30881019

2019

TCTP promotes epithelial-mesenchymal transition in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ruilin Sun,Xi Lu,Li Gong,Faguang Jin

Clinical and translational radiation oncology 15:99-107 PubMed30815593

2019

Assessment of the role of translationally controlled tumor protein 1 (TPT1/TCTP) in breast cancer susceptibility and ATM signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Neuhäuser,Leonie Küper,Hans Christiansen,Natalia Bogdanova

International journal of clinical and experimental 11:2522-2529 PubMed31938365

2018

miR-455-3p functions as a tumor suppressor in colorectal cancer and inhibits cell proliferation by targeting TPT1.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Sun,Yan Wang,Hainan Yang,Yan Xu,Haipeng Yu

Nature communications 8:18 PubMed28550308

2017

Fortilin binds IRE1α and prevents ER stress from signaling apoptotic cell death.

Applications

Species

Unspecified reactive species

Decha Pinkaew,Abhijnan Chattopadhyay,Matthew D King,Preedakorn Chunhacha,Zhihe Liu,Heather L Stevenson,Yanjie Chen,Patuma Sinthujaroen,Owen M McDougal,Ken Fujise

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCTP antibody [EPR5540]

Flow Cytometry (Intracellular) - Anti-TCTP antibody [EPR5540] (AB133568)

Immunocytochemistry/ Immunofluorescence - Anti-TCTP antibody [EPR5540] (AB133568)

Western blot - Anti-TCTP antibody [EPR5540] (AB133568)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (12)

Product promise