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AB133535

Anti-TEF1/TEAD-1 antibody [EPR3968(2)]

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal TEF1/TEAD-1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

대체 명칭 보기

TCF13, TEF1, TEAD1, Transcriptional enhancer factor TEF-1, NTEF-1, Protein GT-IIC, TEA domain family member 1, Transcription factor 13, TEAD-1, TCF-13

4 이미지
Immunocytochemistry/ Immunofluorescence - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (AB133535)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (AB133535)

Immunofluorescence analysis of HeLa cells labelling TEF1/TEAD-1 with ab133535 at 1/50

Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (AB133535)
  • WB

Lab

Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (AB133535)

Lanes 1 - 2 : Merged signal (red and green). Green - ab133535 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab133535 was shown to react with TEF1/TEAD-1 in wild-type A549 cells in western blot with loss of signal observed in TEAD1 knockout sample. Wild-type and TEAD1 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab133535 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

TEAD1 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TEAD1 knockout A549 cell line (<a href='/ko/products/cell-lines/human-tead1-knockout-a549-cell-line-ab261901'>ab261901</a>)

Predicted band size: 48 kDa

Observed band size: 50 kDa

false

Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (AB133535)
  • WB

Unknown

Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (AB133535)

All lanes:

Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

Fetal muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 48 kDa

Observed band size: 52 kDa

false

OI-RD Scanning - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (AB133535)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (AB133535)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3968(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB219647

Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free

RabMAb|Recombinant|KO Validated|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (AB219647)

  • 0
  • 0
Primary Antibodies

AB133533

Anti-TEF1/TEAD-1 antibody [EPR3967(2)]

RabMAb|Recombinant|KO Validated|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] (AB133533)

  • 0
  • 0
Primary Antibodies

AB221367

Anti-TEF1/TEAD-1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TEF1/TEAD-1 antibody (AB221367)

  • 0
  • 0

보완 제품

Primary Antibodies

AB205270

Anti-active YAP1 antibody [EPR19812]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-active YAP1 antibody [EPR19812] (AB205270)

  • 5
  • 25
Primary Antibodies

AB76252

Anti-YAP1 (phospho S127) antibody [EP1675Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)

  • 4
  • 3
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB308621

Anti-TEAD4/TEF-3 antibody [EPR26046-193]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEAD4/TEF-3 antibody [EPR26046-193] (AB308621)

  • 0
  • 0
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Reactivity 정보

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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
See full target information TEAD1
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

MedComm 4:e246 PubMed37197086

2023

YAP/TEAD1 and β-catenin/LEF1 synergistically induce estrogen receptor α to promote osteogenic differentiation of bone marrow stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peiqi Wang,Lingyi Huang,Fan Yang,Wanxi Chen,Ding Bai,Yongwen Guo

The Biochemical journal 475:2955-2967 PubMed30120107

2018

IFN-γ-response mediator GBP-1 represses human cell proliferation by inhibiting the Hippo signaling transcription factor TEAD.

Applications

Unspecified application

Species

Unspecified reactive species

Bea Unterer,Veit Wiesmann,Mekala Gunasekaran,Heinrich Sticht,Clara Tenkerian,Jürgen Behrens,Marina Leone,Felix B Engel,Nathalie Britzen-Laurent,Elisabeth Naschberger,Thomas Wittenberg,Michael Stürzl

American journal of translational research 10:998-1011 PubMed29636889

2018

Effect of TEAD4 on multilineage differentiation of muscle-derived stem cells.

Applications

WB

Species

Cow

Jinze Wang,Feixu Zhang,Huidi Yang,Huikuan Wu,Rong Cui,Yunjie Zhao,Cuihua Jiao,Xianxin Wang,Xin Liu,Liqiong Wu,Guangpeng Li,Xia Wu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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