AB241067

Anti-TEM8/ATR antibody [EPNCI-R173-37]

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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(6 제품이 사용된 논문 )

Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067) is a rabbit monoclonal antibody detecting TEM8/ATR in IHC-P, ICC/IF. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

대체 명칭 보기

ATR, TEM8, ANTXR1, Anthrax toxin receptor 1, Tumor endothelial marker 8

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (AB241067)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (AB241067)

Immunofluorescent analysis of 100% Methanol fixed LoVo (human colorectal adenocarcinoma epithelial cell) cells labeling TEM8/ATR with ab241067 at 1/100 dilution (Green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and plasma membrane staining in LoVo cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

PFA fixation is not recommended due to weak staining on LoVo cells.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (AB241067)

Immunofluorescent analysis of 100% Methanol fixed SW480 (human colorectal adenocarcinoma epithelial cell) cells labeling TEM8/ATR with ab241067 at 1/100 dilution (Green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and plasma membrane staining in SW480 cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

PFA fixation is not recommended due to negative staining on SW480 cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody [EPNCI-R173-37] (AB241067)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody [EPNCI-R173-37] (AB241067)

Immunohistochemical analysis of 10% neutral-buffered formalin Mammary fat pad of TEM8/ATR wildtype (WT) or knockout (KO) athymic nude mice transplanted with HT29 human colon tumor tissue labeling TEM8/ATR with ab241067 at 1/60 dilution, followed by a Biotin anti-rabbit IgG secondsry antibody. Positive staining in stromal cells of transplanted HT29 human colon tumor growing in TEM8/ATR wildtype (WT) athymic nude mice is observed. Antigen retrieval : Retrieved with proteinase K. This image was kindly provided by our collaborator Dr. Brad St. Croix, National Cancer Institute (NCI), NIH, and published in J Clin Invest. 128 : 2927-2943 (PMID : 29863500).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCI-R173-37

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

We have not yet been able to validate ab241067 for IHC-P in-house; however, our collaborator has obtained successful results. Please refer to the protocol and data available in PMID: 29863500.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "(Antigen Retrieval with proteinase K). Perform enzymatic antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "(Antigen Retrieval with proteinase K). Perform enzymatic antigen retrieval before commencing with IHC staining protocol." } } }

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제품 세부 정보

What is this antibody validated in?
Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPNCI-R173-37] also available for your convenience: ab241067, Carrier free - ab246321

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton and thereby plays an important role in normal extracellular matrix (ECM) homeostasis. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. May also act as a receptor for PLAU. Upon ligand binding, stimulates the phosphorylation of EGFR and ERK1/2 (PubMed : 30241478).. (Microbial infection) Acts as a receptor for protective antigen (PA) of B.anthracis.. (Microbial infection) Mediates cell entry of Seneca Valley virus (SVV) when glycosylated.

See full target information ANTXR1

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature cardiovascular research 4:1521-1538 PubMed41039173

2025

ANTXR1 blockade enhances cardiac function in preclinical models of heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola Boccella,GuoJun Yu,Steven Seaman,Yang Feng,Jaewon Lee,Francesco Tomassoni-Ardori,Liping Yang,Kuo-Sheng Hsu,James M Dunleavey,Jodi Becker,Mary Beth Hilton,Karen Morris,Niza Borchin,Daeho So,Pradip Bajgain,Sudhirkumar Yanpallewar,Ryan T Gross,Krish C Dewan,Dawn E Bowles,Darren A Yuen,Lino Tessarollo,Brad St Croix

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 133:e70052 PubMed40671091

2025

Downregulation of lncRNA H19 Enhances Radiosensitivity in Colorectal Cancer by Inactivation of the ATR/CHK1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Liang,Wanlu Zhang,Ao Dungerile,Juping Wang,Runmei Wang

Nature communications 15:1312 PubMed38346978

2024

Residual ANTXR1+ myofibroblasts after chemotherapy inhibit anti-tumor immunity via YAP1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Licaj,Rana Mhaidly,Yann Kieffer,Hugo Croizer,Claire Bonneau,Arnaud Meng,Lounes Djerroudi,Kevin Mujangi-Ebeka,Hocine R Hocine,Brigitte Bourachot,Ilaria Magagna,Renaud Leclere,Lea Guyonnet,Mylene Bohec,Coralie Guérin,Sylvain Baulande,Maud Kamal,Christophe Le Tourneau,Fabrice Lecuru,Véronique Becette,Roman Rouzier,Anne Vincent-Salomon,Geraldine Gentric,Fatima Mechta-Grigoriou

Nature communications 13:7078 PubMed36400786

2022

Cancer cell survival depends on collagen uptake into tumor-associated stroma.

Applications

Unspecified application

Species

Unspecified reactive species

Kuo-Sheng Hsu,James M Dunleavey,Christopher Szot,Liping Yang,Mary Beth Hilton,Karen Morris,Steven Seaman,Yang Feng,Emily M Lutz,Robert Koogle,Francesco Tomassoni-Ardori,Saurabh Saha,Xiaoyan M Zhang,Enrique Zudaire,Pradip Bajgain,Joshua Rose,Zhongyu Zhu,Dimiter S Dimitrov,Frank Cuttitta,Nancy J Emenaker,Lino Tessarollo,Brad St Croix

Cells 11: PubMed36359803

2022

Hypoxia-Regulated lncRNA USP2-AS1 Drives Head and Neck Squamous Cell Carcinoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jianmin Tang,Zheng Wu,Xiaohang Wang,Yanli Hou,Yongrui Bai,Ye Tian

The Journal of clinical investigation 128:2927-2943 PubMed29863500

2018

Tumor stroma-targeted antibody-drug conjugate triggers localized anticancer drug release.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Szot,Saurabh Saha,Xiaoyan M Zhang,Zhongyu Zhu,Mary Beth Hilton,Karen Morris,Steven Seaman,James M Dunleavey,Kuo-Sheng Hsu,Guo-Jun Yu,Holly Morris,Deborah A Swing,Diana C Haines,Yanping Wang,Jennifer Hwang,Yang Feng,Dean Welsch,Gary DeCrescenzo,Amit Chaudhary,Enrique Zudaire,Dimiter S Dimitrov,Brad St Croix

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TEM8/ATR antibody [EPNCI-R173-37]

Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (AB241067)

Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (AB241067)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody [EPNCI-R173-37] (AB241067)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (6)

Product promise